Functional mechanisms of drought tolerance in subtropical maize (Zea mays L.) identified using genome-wide association mapping

BMC Genomics

Hari Shankar Gupta⁴  Trilochan Mohapatra⁵  Trushar Shah²  Abhishek Rathore²  Sumalini Katragadda¹  Tikka Shobha Rani³  Sreelatha Dogga³  Pottekatt Mohanlal Namratha⁴  Sweta Mohan⁴  Swati Mittal⁴  Kaliyugam Shiriga⁴  Rinku Sharma⁴  Kanika Arora⁴  Firoz Hossain⁴  Nepolean Thirunavukkarasu⁴ 

[1] Agricultural Research Station, Acharya N G Ranga Agricultural University, Karimnagar 505001, India;International Crops Research Institute for the Semi-Arid Tropics, Patancheru 502324, India;Maize Research Centre, ARI, Acharya N G Ranga Agricultural University, Rajendra Nagar, Hyderabad 500030, India;Division of Genetics, Indian Agricultural Research Institute, Pusa, New Delhi 110012, India;National Research Centre on Plant Biotechnology, Pusa, New Delhi 110012, India

关键词: Maize;    Drought tolerance;    Water stress;    Candidate SNPs;    Functional mechanisms;    Association mapping;    Genome-wide SNPs;   

Others  :  1118397
DOI  :  10.1186/1471-2164-15-1182

 received in 2014-10-09, accepted in 2014-12-16,  发布年份 2014

【 摘 要 】

Background

Earlier studies were focused on the genetics of temperate and tropical maize under drought. We identified genetic loci and their association with functional mechanisms in 240 accessions of subtropical maize using a high-density marker set under water stress.

Results

Out of 61 significant SNPs (11 were false-discovery-rate-corrected associations), identified across agronomic traits, models, and locations by subjecting the accessions to water stress at flowering stage, 48% were associated with drought-tolerant genes. Maize gene models revealed that SNPs mapped for agronomic traits were in fact associated with number of functional traits as follows: stomatal closure, 28; flowering, 15; root development, 5; detoxification, 4; and reduced water potential, 2. Interactions of these SNPS through the functional traits could lead to drought tolerance. The SNPs associated with ABA-dependent signalling pathways played a major role in the plant’s response to stress by regulating a series of functions including flowering, root development, auxin metabolism, guard cell functions, and scavenging reactive oxygen species (ROS). ABA signalling genes regulate flowering through epigenetic changes in stress-responsive genes. ROS generated by ABA signalling are reduced by the interplay between ethylene, ABA, and detoxification signalling transductions. Integration of ABA-signalling genes with auxin-inducible genes regulates root development which in turn, maintains the water balance by regulating electrochemical gradient in plant.

Conclusions

Several genes are directly or indirectly involved in the functioning of agronomic traits related to water stress. Genes involved in these crucial biological functions interacted significantly in order to maintain the primary as well as exclusive functions related to coping with water stress. SNPs associated with drought-tolerant genes involved in strategic biological functions will be useful to understand the mechanisms of drought tolerance in subtropical maize.

【 授权许可】

   
2014 Thirunavukkarasu et al.; licensee BioMed Central.

【 图 表 】

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