Impact of MBL and MASP-2 gene polymorphism and its interaction on susceptibility to tuberculosis

BMC Infectious Diseases

Shi Wu Wen²  Hongzhuan Tan³  Xin Huang³  Benjamin Kwaku Abuaku¹  Li Hu³  Mian Wang³  Wufei Li⁵  Ying Liang⁴  Mengshi Chen⁶ 

[1] Department of Epidemiology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Legon, Accra, Ghana;Department of Obstetrics & Gynecology, University of Ottawa, The Ottawa Hospital, 501 Smyth Road, Ottawa, Ontario, Canada;Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410008, Hunan, PR China;School of Public Health, Xinjiang Medical University, Urumqi 830054, Xijiang, PR China;Department of Nursing, Shaoyang Medical College, Shaoyang 422000, Hunan, PR China;Hunan Children’s Hospital, Ziyuan RD 86, Changsha 410007, Hunan, PR China

关键词: RERI;    Interaction;    Gene;    MASP-2;    MBL;    Tuberculosis;   

Others  :  1173934
DOI  :  10.1186/s12879-015-0879-y

 received in 2014-01-08, accepted in 2015-03-10,  发布年份 2015

【 摘 要 】

Background

Mannose-binding lectin (MBL) and MBL-associated serine proteases 2 (MASP-2) are important proteins in the lectin pathway of the immune system. Polymorphism of MBL and MASP-2 genes may affect the serum concentration of MBL and MASP-2. This study explores the association between MBL and MASP-2 gene polymorphism and their interactions and the susceptibility to tuberculosis (TB).

Method

A total of 503 patients with TB and 419 healthy controls were recruited to participate in this case-control study. PCR-SSP technology was applied to genotype rs7096206 of MBL genes and rs2273346 and rs6695096 of MASP-2 genes. Demographic data and some exposure information were also obtained from study participants. Unconditional logistic regression analysis was used to identify association between the various factors and TB whilst Marginal Structural Linear Odds Models were used to estimate the interactions.

Results

Both genotype GC at rs7096206 of MBL genes and genotype TC at rs2273346 and rs6695096 of MASP-2 genes were more prevalent in the TB patient group than the healthy control group (P < 0.05, OR 1.393, 1.302 and 1.426 respectively). The relative excess risk of interaction (RERI) between rs7096206 of MBL genes and rs2273346 and rs6695096 of MASP-2 genes was 0.897 (95% CI: 0.282, 1.513) and 1.142 (95% CI: 0.755, 1.530) respectively (P < 0.05).

Conclusion

Polymorphisms of MBL (rs7096206) and MASP-2 (rs2273346 and rs6695096) were associated with the susceptibility of TB, and there were gene-gene interactions among them.

【 授权许可】

   
2015 Chen et al.; licensee BioMed Central.

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