Analysis of T-DNA alleles of flavonoid biosynthesis genes in Arabidopsis ecotype Columbia

BMC Research Notes

Brenda SJ Winkel³  Richard F Helm²  Michelle B Price⁵  Melissa V Ramirez⁴  Peter A Bowerman¹ 

[1] Current address: Department of Biology, Colorado State University, Fort Collins, CO, 80523, USA;Department of Biochemistry, Virginia Tech, Blacksburg, VA, 24061, USA;Department of Biological Sciences, Blacksburg, VA, 24061, USA;Current address: Department of Microbiology, Immunology & Pathology, Colorado State University, Fort Collins, CO, 80523, USA;Current address: Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA, 24061, USA

关键词: Transparent testa;    Flavonoid;    Insertional inactivation lines;    Ecotype;    Arabidopsis;   

Others  :  1165772
DOI  :  10.1186/1756-0500-5-485

 received in 2012-04-27, accepted in 2012-08-23,  发布年份 2012

【 摘 要 】

Background

The flavonoid pathway is a long-standing and important tool for plant genetics, biochemistry, and molecular biology. Numerous flavonoid mutants have been identified in Arabidopsis over the past several decades in a variety of ecotypes. Here we present an analysis of Arabidopsis lines of ecotype Columbia carrying T-DNA insertions in genes encoding enzymes of the central flavonoid pathway. We also provide a comprehensive summary of various mutant alleles for these structural genes that have been described in the literature to date in a wide variety of ecotypes.

Findings

The confirmed knockout lines present easily-scorable phenotypes due to altered pigmentation of the seed coat (or testa). Knockouts for seven alleles for six flavonoid biosynthetic genes were confirmed by PCR and characterized by UPLC for altered flavonol content.

Conclusion

Seven mutant lines for six genes of the central flavonoid pathway were characterized in ecotype, Columbia. These lines represent a useful resource for integrating biochemical and physiological studies with genomic, transcriptomic, and proteomic data, much of which has been, and continues to be, generated in the Columbia background.

【 授权许可】

   
2012 Bowerman et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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