Development and characterization of 16 polymorphic microsatellite markers from Taiwan cow-tail fir, Keteleeria davidiana var. formosana (Pinaceae) and cross-species amplification in other Keteleeria taxa

BMC Research Notes

Yu-Chung Chiang⁴  Tsai-Wen Hsu⁵  Chaur-Tzuhn Chen²  Yu-Shen Shih⁷  Ya-Zhu Ko⁴  Li-Ping Ju⁹  Mei-Hui Chen⁶  Shau-Ting Chiu³  Ho-Yih Liu⁴  Huei-Chuan Shih¹  Chin-Shang Ho⁸ 

[1] Department of Nursing, Meiho University, Pingtung 912, Taiwan;Department of Forestry, National Pingtung University of Science and Technology, Pingtung 912, Taiwan;Department of Biology, National Museum of Natural Science, Taichung 404, Taiwan;Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan;Endemic Species Research Institute, Nantou 552, Taiwan;Conservation Division, Forestry Bureau, Council of Agriculture, Executive Yuan, Taipei 100, Taiwan;Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan;Graduate Institute of Bioresources, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan;Botanical Garden Division, Taiwan Forestry Research Institute, Taipei 100, Taiwan

关键词: Pinaceae;    Simple sequence repeat markers;    Microsatellites;    Conservation;    Keteleeria;   

Others  :  1133264
DOI  :  10.1186/1756-0500-7-255

 received in 2014-02-13, accepted in 2014-04-16,  发布年份 2014

【 摘 要 】

Background

Keteleeria davidiana var. formosana (Pinaceae), Taiwan cow-tail fir, is an endangered species listed on the IUCN Red List of Threatened Species and only two populations remain, both on the Taiwan Island. Sixteen polymorphic microsatellite loci were developed in an endangered and endemic gymnosperm species, Keteleeria davidiana var. formosana, and were tested in an additional 6 taxa, K. davidiana var. calcarea, K. davidiana var. chienpeii, K. evelyniana, K. fortunei, K. fortunei var. cyclolepis, and K. pubescens, to evaluate the genetic variation available for conservation management and to reconstruct the phylogeographic patterns of this ancient lineage.

Findings

Polymorphic primer sets were developed from K. davidiana var. formosana using the modified AFLP and magnetic bead enrichment method. The number of alleles ranged from 3 to 16, with the observed heterozygosity ranging from 0.28 to 1.00. All of the loci were found to be interspecifically amplifiable.

Conclusions

These polymorphic and transferable loci will be potentially useful for future studies that will focus on identifying distinct evolutionary units within species and establishing the phylogeographic patterns and the process of speciation among closely related species.

【 授权许可】

   
2014 Ho et al.; licensee BioMed Central Ltd.

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