Post-weaning selenium and folate supplementation affects gene and protein expression and global DNA methylation in mice fed high-fat diets

BMC Medical Genomics

Matthew PG Barnett⁴  William A Laing¹  Di T Brewster¹  Janine M Cooney³  Warren C McNabb⁵  Nicole C Roy²  Wayne Young⁴  Shalome A Bassett⁴  Emma N Bermingham⁴ 

[1]Biological Chemistry & Bioactives, Food Innovation, Plant & Food Research Mt Albert, Auckland 1025, New Zealand

[2]Riddet Institute, Massey University, Palmerston North 4442, New Zealand

[3]Biological Chemistry & Bioactives, Food Innovation, Plant & Food Research Ruakura, Hamilton 3240, New Zealand

[4]Food Nutrition & Health Team, Food & Bio-based Products Group, AgResearch Grasslands, Palmerston North 4442, New Zealand

[5]AgResearch Grasslands, Palmerston North, 4442 New Zealand

关键词: High fat;    Selenium;    Folate;    Proteomics;    2D-DIGE;    Microarray analysis;    Epigenetic;   

Others  :  1121164
DOI  :  10.1186/1755-8794-6-7

 received in 2012-06-11, accepted in 2013-02-18,  发布年份 2013

【 摘 要 】

Background

Consumption of high-fat diets has negative impacts on health and well-being, some of which may be epigenetically regulated. Selenium and folate are two compounds which influence epigenetic mechanisms. We investigated the hypothesis that post-weaning supplementation with adequate levels of selenium and folate in offspring of female mice fed a high-fat, low selenium and folate diet during gestation and lactation will lead to epigenetic changes of potential importance for long-term health.

Methods

Female offspring of mothers fed the experimental diet were either maintained on this diet (HF-low-low), or weaned onto a high-fat diet with sufficient levels of selenium and folate (HF-low-suf), for 8 weeks. Gene and protein expression, DNA methylation, and histone modifications were measured in colon and liver of female offspring.

Results

Adequate levels of selenium and folate post-weaning affected gene expression in colon and liver of offspring, including decreasing Slc2a4 gene expression. Protein expression was only altered in the liver. There was no effect of adequate levels of selenium and folate on global histone modifications in the liver. Global liver DNA methylation was decreased in mice switched to adequate levels of selenium and folate, but there was no effect on methylation of specific CpG sites within the Slc2a4 gene in liver.

Conclusions

Post-weaning supplementation with adequate levels of selenium and folate in female offspring of mice fed high-fat diets inadequate in selenium and folate during gestation and lactation can alter global DNA methylation in liver. This may be one factor through which the negative effects of a poor diet during early life can be ameliorated. Further research is required to establish what role epigenetic changes play in mediating observed changes in gene and protein expression, and the relevance of these changes to health.

【 授权许可】

   
2013 Bermingham et al; licensee BioMed Central Ltd.

【 图 表 】

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