Tea polyphenols alleviate high fat and high glucose-induced endothelial hyperpermeability by attenuating ROS production via NADPH oxidase pathway

BMC Research Notes

Xiaolei Ye⁴  Chenjiang Ying²  Shibin Ding²  Ying Zhang²  Xin Jin²  Yi Meng²  Weiye Ren²  Nana Zhao¹  Chong Tian³  Xuezhi Zuo⁵ 

[1] School of Environmental Science and Public Health, Wenzhou Medical University, Wenzhou 325035, PR China;Department of Nutrition and Food Hygiene and MOE Key Laboratory of Environment and Health, School of Public health, Huazhong University of Science & Technology, Wuhan 430030, PR China;School of Nursing, Tongji medical college, Huazhong University of Science & Technology, Wuhan 430030, PR China;Present address: School of Public Health, Wenzhou Medical University, WenZhou 325035, PR China;Department of Clinical Nutrition, Tongji Hospital, Huazhong University of Science & Technology, Wuhan 430030, PR China

关键词: NADPH oxidase;    Hyperpermeability;    High glucose;    High fat;    Green tea polyphenols;   

Others  :  1134396
DOI  :  10.1186/1756-0500-7-120

 received in 2013-09-28, accepted in 2014-02-24,  发布年份 2014

【 摘 要 】

Background

Hyperglycemia-induced endothelial hyperpermeability is crucial to cardiovascular disorders and macro-vascular complications in diabetes mellitus. The objective of this study is to investigate the effects of green tea polyphenols (GTPs) on endothelial hyperpermeability and the role of nicotinamide adenine dinucleotide phosphate (NADPH) pathway.

Methods

Male Wistar rats fed on a high fat diet (HF) were treated with GTPs (0, 0.8, 1.6, 3.2 g/L in drinking water) for 26 weeks. Bovine aortic endothelial cells (BAECs) were treated with high glucose (HG, 33 mmol/L) and GTPs (0.0, 0.4, or 4 μg/mL) for 24 hours in vitro. The endothelial permeabilities in rat aorta and monolayer BAECs were measured by Evans blue injection method and efflux of fluorescein isothiocyanate (FITC)-dextran, respectively. The reactive oxygen species (ROS) levels in rat aorta and monolayer BAECs were measured by dihydroethidium (DHE) and 2′, 7′-dichloro-fluorescein diacetate (DCFH-DA) fluorescent probe, respectively. Protein levels of NADPH oxidase subunits were determined by Western-blot.

Results

HF diet-fed increased the endothelial permeability and ROS levels in rat aorta while HG treatments increased the endothelial permeability and ROS levels in cultured BAECs. Co-treatment with GTPs alleviated those changes both in vivo and in vitro. In in vitro studies, GTPs treatments protected against the HG-induced over-expressions of p22^phox and p67^phox. Diphenylene iodonium chloride (DPI), an inhibitor of NADPH oxidase, alleviated the hyperpermeability induced by HG.

Conclusions

GTPs could alleviate endothelial hyperpermeabilities in HF diet-fed rat aorta and in HG treated BAECs. The decrease of ROS production resulting from down-regulation of NADPH oxidase contributed to the alleviation of endothelial hyperpermeability.

【 授权许可】

   
2014 Zuo et al.; licensee BioMed Central Ltd.

【 图 表 】

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