Evaluation of apocynin in vitro on high glucose-induced oxidative stress on tenocytes

Bone & Joint Research

article

Takashi Kurosawa¹  Yutaka Mifune¹  Atsuyuki Inui¹  Hanak Nishimoto¹  Yasuhiro Ueda¹  Takeshi Kataoka¹  Kohei Yamaura¹  Shintaro Mukohara¹  Ryosuke Kuroda¹ 

[1] Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine

关键词: High glucose;    Oxidative stress;    Apocynin;    NADPH oxidase;    Diabetic tendinopathy;   

DOI  :  10.1302/2046-3758.991.BJR-2019-0074.R1

学科分类：骨科学

来源: British Editorial Society Of Bone And Joint Surgery

【 摘 要 】

AimsThe purpose of this study was to evaluate the in vitro effects of apocynin, an inhibitor of nicotinamide adenine dinucleotide phosphate oxidase (NOX) and a downregulator of intracellular reactive oxygen species (ROS), on high glucose-induced oxidative stress on tenocytes.MethodsTenocytes from normal Sprague-Dawley rats were cultured in both control and high-glucose conditions. Apocynin was added at cell seeding, dividing the tenocytes into four groups: the control group; regular glucose with apocynin (RG apo+); high glucose with apocynin (HG apo+); and high glucose without apocynin (HG apo–). Reactive oxygen species production, cell proliferation, apoptosis and messenger RNA (mRNA) expression of NOX1 and 4, and interleukin-6 (IL-6) were determined in vitro.ResultsExpression of NOX1, NOX4, and IL-6 mRNA in the HG groups was significantly higher compared with that in the RG groups, and NOX1, NOX4, and IL-6 mRNA expression in the HG apo+ group was significantly lower compared with that in the HG apo– group. Cell proliferation in the RG apo+ group was significantly higher than in the control group and was also significantly higher in the HG apo+ group than in the HG apo– group. Both the ROS accumulation and the amounts of apoptotic cells in the HG groups were greater than those in the RG groups and were significantly less in the HG apo+ group than in the HG apo– group.ConclusionApocynin reduced ROS production and cell death via NOX inhibition in high-glucose conditions. Apocynin is therefore a potential prodrug in the treatment of diabetic tendinopathy.

【 授权许可】

CC BY-NC