Comprehensive annotation of microRNA expression profiles

BMC Genetics

Zhong-Dang Xiao¹  Ning-Ping Huang¹  Fei-Hu Hu¹  Fei Yang¹  Bo Sun¹ 

[1] State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China

关键词: Comprehensive annotation;    Deep sequencing;    Microarray;    MicroRNA;   

Others  :  1086125
DOI  :  10.1186/1471-2156-14-120

 received in 2013-07-19, accepted in 2013-11-26,  发布年份 2013

【 摘 要 】

Background

MicroRNAs (miRNAs) regulate many biological processes by post-translational gene silencing. Analysis of miRNA expression profiles is a reliable method for investigating particular biological processes due to the stability of miRNA and the development of advanced sequencing methods. However, this approach is limited by the broad specificity of miRNAs, which may target several mRNAs.

Result

In this study, we developed a method for comprehensive annotation of miRNA array or deep sequencing data for investigation of cellular biological effects. Using this method, the specific pathways and biological processes involved in Alzheimer’s disease were predicted with high correlation in four independent samples. Furthermore, this method was validated for evaluation of cadmium telluride (CdTe) nanomaterial cytotoxicity. As a result, apoptosis pathways were selected as the top pathways associated with CdTe nanoparticle exposure, which is consistent with previous studies.

Conclusions

Our findings contribute to the validation of miRNA microarray or deep sequencing results for early diagnosis of disease and evaluation of the biological safety of new materials and drugs.

【 授权许可】

   
2013 Sun et al.; licensee BioMed Central Ltd.

【 图 表 】

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