Regulation of the microtubular cytoskeleton by Polycystin-1 favors focal adhesions turnover to modulate cell adhesion and migration

BMC Cell Biology

Alessandra Boletta⁴  Letizia Lanzetti³  Amanda Oldani²  Nadia Ducano³  Gianfranco Distefano⁴  Chiara De Pascalis¹  Maddalena Castelli⁴ 

[1]Current Address: International PhD Program, Institut Pasteur, Paris, France

[2]IFOM, Fondazione Istituto FIRC di Oncologia Molecolare, Milan, Italy

[3]Department of Oncology, University of Torino, Torino, Italy

[4]Division of Genetics and Cell Biology, San Raffaele Scientific Institute, Milan, Italy

关键词: Focal adhesion kinase;    Focal adhesion turnover;    Adhesion;    Microtubules;    Migration;    Polycystin-1;   

Others  :  1211779
DOI  :  10.1186/s12860-015-0059-3

 received in 2015-02-04, accepted in 2015-04-22,  发布年份 2015

【 摘 要 】

Background

Polycystin-1 (PC-1) is a large plasma membrane receptor, encoded by the PKD1 gene, which is mutated in most cases of Autosomal Dominant Polycystic Kidney Disease (ADPKD). The disease is characterized by renal cysts. The precise function of PC-1 remains elusive, although several studies suggest that it can regulate the cellular shape in response to external stimuli. We and others reported that PC-1 regulates the actin cytoskeleton and cell migration.

Results

Here we show that cells over-expressing PC-1 display enhanced adhesion rates to the substrate, while cells lacking PC-1 have a reduced capability to adhere. In search for the mechanism responsible for this new property of PC-1 we found that this receptor is able to regulate the stability of the microtubules, in addition to its capability to regulate the actin cytoskeleton. The two cytoskeletal components are acting in a coordinated fashion. Notably, we uncovered that PC-1 regulation of the microtubule cytoskeleton impacts on the turnover rates of focal adhesions in migrating cells and we link all these properties to the capability of PC-1 to regulate the activation state of Focal Adhesion Kinase (FAK).

Conclusions

In this study we show several new features of the PC-1 receptor in modulating microtubules and adhesion dynamics, which are essential for its capability to regulate migration.

【 授权许可】

   
2015 Castelli et al.; licensee BioMed Central.

【 图 表 】

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