A possible anti-inflammatory mechanism of ethyl acetate extracts of Teucrium stocksianum Bioss

BMC Complementary and Alternative Medicine

Syed Muhammad Mukarram Shah¹ 

[1]Department of Pharmacy, University of Malakand, Chakdara, Dir, Pakistan

关键词: Arachedonic acid;    Bradykinin;    Histamine;    Anti-inflammatory mechanism;    Teucrium stocksianum;   

Others  :  1223285
DOI  :  10.1186/s12906-015-0834-x

 received in 2015-06-15, accepted in 2015-08-26,  发布年份 2015

【 摘 要 】

Background

Teucrium stocksianum (T. stocksianum) is one of the important members of the genus Teucrium which contains numerous biologically active compounds. Traditionally, it is used for the treatment of fever, pain, as expectorant and blood purifier. Researchers are trying to discover plants origin, novel and safe remedies for the management of various ailments. The present study was aimed to determine the possible anti-inflammatory mechanism of ethyl acetate extract of T. stocksianum.

Methods

Preliminary, the ethanolic extract and sub-fractions were screened for anti-inflammatory potential at doses of 100, 200 and 300 mg/kg (i.p) body weight, using carrageenan induced paw edema test in mice. In-order to determine the possible mechanism of anti-inflammatory effect, the ethyl acetate fraction was ascertained with different phlogistic agents like histamine, bradykinin, prostaglandins E ₂and arachedonic acid via paw edema test in mice.

Results

The ethanolic extract and sub-fractions of T. stocksianum displayed marked to moderate anti-inflammatory activity in a carrageenan induced paw edema test in mice. Among the sub-fractions, ethyl acetate fraction (EAF) demonstrated excellent (66 %) anti-inflammatory action at the highest tested dose (300 mg/kg) that reached to the maximum value at 3 ^rdhour after carrageenan injection and remained significant (***P < 0.001) till 5 ^thhour of test sample administration. EAF revealed moderate effect against the paw edema induced by histamine (31.048 %) while non-significant results (18.148 %) were observed against the edema induced by bradykinin. The extract demonstrated significant (66.23-73.076 %) anti-inflammatory potential against the edematogenic effect of prostaglandin E ₂ . Moreover, the extract also significantly inhibited (51.33 %) the paw edema induced by arachedonic acid.

Conclusion

Our results suggest that the EAF has dual action and produced the anti-inflammatory effect by blocking both pathways of arachedonic acid metabolites (cyclooxygenase and lipoxygenase). Thus validating the traditional use of T. stocksianum and could provide a source of novel, effective and safe drug for the treatment of inflammation.

【 授权许可】

   
2015 Mukarram Shah.

【 图 表 】

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