Classification of fungal and bacterial lytic polysaccharide monooxygenases

BMC Genomics

Lene Lange¹  Peter K Busk¹ 

[1] Department of Chemistry and Bioscience, Aalborg University and Barentzymes A/S, A.C. Meyers Vænge 15, 2450, Copenhagen, SV, Denmark

关键词: Genomic annotation;    Peptide pattern recognition;    Sequence analysis;    Subfamilies;    Lytic polysaccharide monooxygenases;   

Others  :  1204009
DOI  :  10.1186/s12864-015-1601-6

 received in 2014-11-04, accepted in 2015-04-29,  发布年份 2015

【 摘 要 】

Background

Lytic polysaccharide monooxygenases are important enzymes for the decomposition of recalcitrant biological macromolecules such as plant cell wall and chitin polymers. These enzymes were originally designated glycoside hydrolase family 61 and carbohydrate-binding module family 33 but are now classified as auxiliary activities 9, 10 and 11 in the CAZy database. To obtain a systematic analysis of the divergent families of lytic polysaccharide monooxygenases we used Peptide Pattern Recognition to divide 5396 protein sequences resembling enzymes from families AA9 (1828 proteins), AA10 (2799 proteins) and AA11 (769 proteins) into subfamilies.

Results

The results showed that the lytic polysaccharide monooxygenases have two conserved regions identified by conserved peptides specific for each AA family. The peptides were used for in silico PCR discovery of the lytic polysaccharide monooxygenases in 79 fungal and 95 bacterial genomes. The bacterial genomes encoded 0 – 7 AA10s (average 0.6). No AA9 or AA11 were found in the bacteria. The fungal genomes encoded 0 – 40 AA9s (average 7) and 0 – 15 AA11s (average 2) and two of the fungi possessed a gene encoding a putative AA10. The AA9s were mainly found in plant cell wall-degrading asco- and basidiomycetes in agreement with the described role of AA9 enzymes. In contrast, the AA11 proteins were found in 36 of the 39 ascomycetes and in only two of the 32 basidiomycetes and their abundance did not correlate to the degradation of cellulose and hemicellulose.

Conclusions

These results provides an overview of the sequence characteristics and occurrence of the divergent AA9, AA10 and AA11 families and pave the way for systematic investigations of the of lytic polysaccharide monooxygenases and for structure-function studies of these enzymes.

【 授权许可】

   
2015 Busk and Lange; licensee BioMed Central.

【 图 表 】

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