Genome-wide association study of antibody response to Newcastle disease virus in chicken

BMC Genetics

Dingming Shu¹  Ning Li²  Xiaoxiang Hu²  Chunfen Yang¹  Chunyu Li¹  Jie Wang¹  Jie Ma¹  Hao Qu¹  Chenglong Luo¹ 

[1] State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, 510640, China;State Key Laboratory for Agro-Biotechnology, China Agricultural University, Beijing, 100193, China

关键词: Genome-wide association study;    Antibody response;    Newcastle disease;    Chicken;   

Others  :  1087070
DOI  :  10.1186/1471-2156-14-42

 received in 2012-09-01, accepted in 2013-05-06,  发布年份 2013

【 摘 要 】

Background

Since the first outbreak in Indonesia in 1926, Newcastle disease has become one of the most common and contagious bird diseases throughout the world. To date, enhancing host antibody response by vaccination remains the most efficient strategy to control outbreaks of Newcastle disease. Antibody response plays an important role in host resistance to Newcastle disease, and selection for antibody response can effectively improve disease resistance in chickens. However, the molecular basis of the variation in antibody response to Newcastle disease virus (NDV) is not clear. The aim of this study was to detect genes modulating antibody response to NDV by a genome-wide association study (GWAS) in chickens.

Results

To identify genes or chromosomal regions associated with antibody response to NDV after immunization, a GWAS was performed using 39,833 SNP markers in a chicken F₂ resource population derived from a cross between two broiler lines that differed in their resistance. Two SNP effects reached 5% Bonferroni genome-wide significance (P<1.26×10^-6). These two SNPs, rs15354805 and rs15355555, were both on chicken (Gallus gallus) chromosome 1 and spanned approximately 600 Kb, from 100.4 Mb to 101.0 Mb. Rs15354805 is in intron 7 of the chicken Roundabout, axon guidance receptor, homolog 2 (ROBO2) gene, and rs15355555 is located about 243 Kb upstream of ROBO2. Rs15354805 explained 5% of the phenotypic variation in antibody response to NDV, post immunization, in chickens. Rs15355555 had a similar effect as rs15354805 because of its linkage disequilibrium with rs15354805 (r²=0.98).

Conclusion

The region at about 100 Mb from the proximal end of chicken chromosome 1, including the ROBO1 and ROBO2 genes, has a strong effect on the antibody response to the NDV in chickens. This study paves the way for further research on the host immune response to NDV.

【 授权许可】

   
2013 Luo et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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