Expression of antigen tf and galectin-3 in fibroadenoma

BMC Research Notes

Pedro Hernández³  Maria del Socorro Pina³  Edgar Zenteno¹  Sergio Aguilar³  Laura Pérez³  Miguel Ángel Mayoral³  Margarito Martinez²  Eduardo Pérez-Campos²  Itandehui Belem Gallegos³ 

[1] Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico, 04510, Mexico;Unidad de Investigación en Bioquímica, Instituto Tecnológico de Oaxaca, Oaxaca, Mexico;Centro de Investigaciones en Ciencias Medicas y Biológicas Facultad de Medicina, Universidad Autónoma Benito Juárez de Oaxaca, 68020, Oaxaca, Mexico

关键词: Plant lectins;    Breast cancer;    Fibroadenoma;    Galectin-3;    Antigen TF;   

Others  :  1165017
DOI  :  10.1186/1756-0500-5-694

 received in 2012-08-30, accepted in 2012-12-18,  发布年份 2012

【 摘 要 】

Background

Fibroadenomas are benign human breast tumors, characterized by proliferation of epithelial and stromal components of the terminal ductal unit. They may grow, regress or remain unchanged, as the hormonal environment of the patient changes. Expression of antigen TF in mucin or mucin-type glycoproteins and of galectin-3 seems to contribute to proliferation and transformations events; their expression has been reported in ductal breast cancer and in aggressive tumors.

Findings

Lectin histochemistry, immunohistochemistry, and immunofluorescence were used to examine the expression and distribution of antigen TF and galectin-3. We used lectins from Arachis hypogaea, Artocarpus integrifolia, and Amaranthus lecuocarpus to evaluate TF expression and a monoclonal antibody to evaluate galectin-3 expression. We used paraffin-embedded blocks from 10 breast tissues diagnosed with fibroadenoma and as control 10 healthy tissue samples. Histochemical and immunofluorescence analysis showed positive expression of galectin-3 in fibroadenoma tissue, mainly in stroma, weak interaction in ducts was observed; whereas, in healthy tissue samples the staining was also weak in ducts. Lectins from A. leucocarpus and A. integrifolia specificaly recognized ducts in healthy breast samples, whereas the lectin from A. hypogaea recognized ducts and stroma. In fibroadenoma tissue, the lectins from A. integrifolia, A. Hypogaea, and A. leucocarpus recognized mainly ducts.

Conclusions

Our results suggest that expression of antigen TF and galectin-3 seems to participate in fibroadenoma development.

【 授权许可】

   
2012 Gallegos et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

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