Serological and molecular detection of Toscana and other Phleboviruses in patients and sandflies in Tunisia

BMC Infectious Diseases

Ali Bouattour⁴  Maria Grazia Cusi¹  Henda Triki³  Nahed Hogga³  Lamia Ammari²  Gianni Gori Savellini¹  Youmna M’ghirbi⁴  Ons Fezaa⁴ 

[1] Department of Medical Biotechnologies, University of Siena, Policlinico Le Scotte, Siena, Italy;Service de Maladies Infectieuses, CHU la Rabta Tunis, Faculté de Médecine Tunis, Université Tunis El Manar, Tunis, Tunisia;Laboratoire de Recherche Diversité Génétique des Entérovirus et Virus des Hépatites, Institut Pasteur de Tunis, Faculté de Médecine de Tunis, Université Tunis El Manar, Tunis, Tunisia;Laboratoire d’Epidémiologie et Microbiologie Vétérinaire, Service d’Entomologie Médicale, Institut Pasteur de Tunis, Université Tunis El Manar, Tunis, Tunisia

关键词: Tunisia;    Phlebotomus;    Sandflies;    Phlebovirus;    Punique virus;    Sandfly Fever Sicilian virus;    Viral isolation;    Nested RT-PCR;    ELISA;    Toscana virus;   

Others  :  1122030
DOI  :  10.1186/s12879-014-0598-9

 received in 2014-06-16, accepted in 2014-10-29,  发布年份 2014

【 摘 要 】

Background

Our aim is to detect the infection by Toscana virus (TOSV) and other Phleboviruses in the sera and cerebro-spinal fluid (CSF) of patients with meningitis in Tunisia. We examined various species of phlebotomus present in Tunisia to determine whether or not a direct relationship exists between cases of meningitis and the viruses circulating in the insect vectors.

Methods

Patients with the meningeal syndrome were tested for anti-TOSV IgM and IgG using an indirect Enzyme-Linked Immunosorbent Assay (ELISA) and for the presence of TOSV and other Phleboviruses using a RT-PCR test.

An entomological study was carried out using CDC light traps to trap sandflies in different bioclimatic zones of Tunisia. Collected sandflies were tested by RT-PCR for the presence of TOSV and other Phleboviruses and subsequently by viral isolation on Vero cells.

Results

Of 263 patients were tested using ELISA of which 12.16% (n = 32/263) were IgM positive for anti TOSV. Of these 32 patients, 78% (n = 25/32) were IgG positive. 12.86% (n = 18/140) of the CSF samples tested by RT-PCR were positive for the Toscana virus.

One CSF sample tested by RT-PCR revealed the presence of Sandfly Fever Sicilian Virus (SFSV). The Punique virus was identified in one sandfly pool.

Conclusions

This study confirms, for the first time, that TOSV is involved in a neurological disorder in North Africa. The incidence of this involvement in Tunisia conforms with observations made in other Mediterranean countries. Moreover, for the first time, a molecular approach was used to detect SFSV in a Tunisian patient displaying neurological symptoms.

【 授权许可】

   
2014 Fezaa et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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