Mutagenicity and antimutagenicity of (−)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays

BMC Complementary and Alternative Medicine

Eliana Aparecida Varanda¹  Márcio Luis de Andrade e Silva²  Karen Cristina de Souza Rezende²  Mariana Santoro de Camargo¹  Denise Crispim Tavares²  Lilian Cristina Barbosa²  Flávia Aparecida Resende¹ 

[1] Departamento de Ciências Biológicas, UNESP-Universidade Estadual Paulista Julio de Mesquita Filho- Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, São Paulo, 14801-902, Brazil;Universidade de Franca, Franca, São Paulo, 14404-600, Brazil

关键词: Antimutagenicity;    Mutagenicity;    Comet assay;    Ames test;    Hinokinin;   

Others  :  1231517
DOI  :  10.1186/1472-6882-12-203

 received in 2012-08-20, accepted in 2012-10-29,  发布年份 2012

【 摘 要 】

Background

The dibenzylbutyrolactone lignan (−)-hinokinin (HK) was derived by partial synthesis from (−)-cubebin, isolated from the dry seeds of the pepper, Piper cubeba. Considering the good trypanosomicidal activity of HK and recalling that natural products are promising starting points for the discovery of novel potentially therapeutic agents, the aim of the present study was to investigate the (anti) mutagenic∕ genotoxic activities of HK.

Methods

The mutagenic∕ genotoxic activities were evaluated by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, and the comet assay, so as to assess the safe use of HK in the treatment of Chagas’ disease. The antimutagenic ∕antigenotoxic potential of HK were also tested against the mutagenicity of a variety of direct and indirect acting mutagens, such as 4- nitro-o-phenylenediamine (NOPD), sodium azide (SA), mitomycin C (MMC), benzo[a]pyrene (B[a]P), aflatoxin B₁ (AFB₁), 2-aminoanthracene (2-AA) and 2-aminofluorene (2-AF), by the Ames test, and doxorubicin (DXR) by the comet assay.

Results

The mutagenicity∕genotoxicity tests showed that HK did not induce any increase in the number of revertants or extent of DNA damage, demonstrating the absence of mutagenic and genotoxic activities. On the other hand, the results on the antimutagenic potential of HK showed a strong inhibitory effect against some direct and indirect-acting mutagens.

Conclusions

Regarding the use of HK as an antichagasic drug, the absence of mutagenic effects in animal cell and bacterial systems is encouraging. In addition, HK may be a new potential antigenotoxic ∕ antimutagenic agent from natural sources. However, the protective activity of HK is not general and varies with the type of DNA damage-inducing agent used.

【 授权许可】

   
2012 Resende et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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