Chemical profile and in vivo hypoglycemic effects of Syzygium jambos, Costus speciosus and Tapeinochilos ananassae plant extracts used as diabetes adjuvants in Puerto Rico

BMC Complementary and Alternative Medicine

Michelle M. Martínez-Montemayor⁶  Luis A. Cubano¹  Gerónimo Maldonado-Martínez⁵  Lorelein Morales-Santiago²  Wanda Figueroa-Cuilan³  Karla Rodríguez-Tirado⁴  Natalie Rivera-Ortiz²  Alexandra Aguilar-Perez⁶  Jannette Gavillán-Suárez² 

[1] Department of Anatomy and Cell Biology, Universidad Central del Caribe-School of Medicine, Bayamón 00960-6032, Puerto Rico;Department of Chemistry, University of Puerto Rico at Cayey, Cayey 00737-2230, Puerto Rico;Present address: University of Missouri-Columbia, Division of Biological Sciences, 110 Tucker Hall, Columbia 65211-7400, MO, USA;Present address: University of Puerto Rico Medical Sciences Campus, San Juan 00936-5067, Puerto Rico;Data Management & Statistical Research Support Unit, Universidad Central del Caribe-School of Medicine, Bayamón 00960-6032, Puerto Rico;Department of Biochemistry, Universidad Central del Caribe-School of Medicine, Bayamón 00960-6032, Puerto Rico

关键词: Costus speciosus;    Tapeinochilus ananassae;    Syzygium jambos;    Chemical profile;    C57BLKS/J db/db and C57BL/J ob/ob mice;    Diabetes adjuvants;   

Others  :  1221184
DOI  :  10.1186/s12906-015-0772-7

 received in 2015-02-06, accepted in 2015-07-08,  发布年份 2015

【 摘 要 】

Background

The increasing numbers of people who use plant-based remedies as alternative or complementary medicine call for the validation of less known herbal formulations used to treat their ailments. Since Puerto Rico has the highest rate of Type 2 diabetes within all the states and territories of the United States, and Puerto Ricans commonly use plants as diabetes adjuvants, it is important to study the plants’ physiological effects, and identify their bioactive compounds to understand their role in modulation of blood glucose levels. We present the phytochemical profiles and hypoglycemic effects of Tapeinochilus ananassae, Costus speciosus and Syzygium jambos.

Methods

Phytochemicals in methanolic and aqueous extracts were analyzed by thin layer chromatography (TLC). Alkaloids (Bromocresol green, λ = 470 nm), flavonoids (AlCl ₃ , λ = 415 nm), saponins (DNS, λ = 760 nm), tannins (FeCl ₃ /K ₄ Fe(CN) ₆ , λ = 395 nm) and phenolics (Folin-Ciocalteau, λ = 765 nm) were quantified. Male C57BLKS/J (db/db) and C57BL/J (ob/ob) genetically obese mice were orally gavaged with aqueous extracts of lyophilized plant decoctions for 10wks.

Results

Our results show that T. ananassae had significantly greater amounts of flavonoids and tannins, while S. jambos showed the greatest concentration of phenolics and C. speciosus exhibited higher amounts of alkaloids. C57BLKS/J db/db treated with plant extracts show better glucose modulation when the extracts are administered in complement with an insulin injection. Finally, C57BL/J ob/ob mice on T. ananassae and S. jambos treatments show better blood glucose modulation over time.

Conclusion

These results document for the first time the chemical profile of T. ananassae and provide evidence for a potential anti-diabetic efficacy of T. ananassae and S. jambos.

【 授权许可】

   
2015 Gavillán-Suárez et al.

【 图 表 】

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