Parallel re-modeling of EF-1α function: divergent EF-1α genes co-occur with EFL genes in diverse distantly related eukaryotes

BMC Evolutionary Biology

Yuji Inagaki¹  Tetsuo Hashimoto¹  Andrew J Roger⁵  Alastair GB Simpson⁶  Ryan Gawryluk⁵  Naoji Yubuki²  Aaron A Heiss⁶  Yoshihiko Sako³  Yuki Nishimura⁴  Matthew W Brown⁵  Ryoma Kamikawa⁷ 

[1] Center for Computational Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8577, Japan;Department of Botany, University of British Columbia, 6270 University Blvd., Vancouver, BC V6T 1Z4, Canada;Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Kyoto 606-8502, Japan;Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan;Centre for Comparative Genomics and Evolutionary Bioinformatics, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS, Canada;Department of Biology, Dalhousie University, Halifax NS, Canada;Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan

关键词: Thecamonas;    Spizellomyces;    Pythium;    Goniomonas;    Functional Remodeling;    EFL;    EF-1α;    Differential Gene Loss;    Diatoms;   

Others  :  1087022
DOI  :  10.1186/1471-2148-13-131

 received in 2013-04-26, accepted in 2013-06-21,  发布年份 2013

【 摘 要 】

Background

Elongation factor-1α (EF-1α) and elongation factor-like (EFL) proteins are functionally homologous to one another, and are core components of the eukaryotic translation machinery. The patchy distribution of the two elongation factor types across global eukaryotic phylogeny is suggestive of a ‘differential loss’ hypothesis that assumes that EF-1α and EFL were present in the most recent common ancestor of eukaryotes followed by independent differential losses of one of the two factors in the descendant lineages. To date, however, just one diatom and one fungus have been found to have both EF-1α and EFL (dual-EF-containing species).

Results

In this study, we characterized 35 new EF-1α/EFL sequences from phylogenetically diverse eukaryotes. In so doing we identified 11 previously unreported dual-EF-containing species from diverse eukaryote groups including the Stramenopiles, Apusomonadida, Goniomonadida, and Fungi. Phylogenetic analyses suggested vertical inheritance of both genes in each of the dual-EF lineages. In the dual-EF-containing species we identified, the EF-1α genes appeared to be highly divergent in sequence and suppressed at the transcriptional level compared to the co-occurring EFL genes.

Conclusions

According to the known EF-1α/EFL distribution, the differential loss process should have occurred independently in diverse eukaryotic lineages, and more dual-EF-containing species remain unidentified. We predict that dual-EF-containing species retain the divergent EF-1α homologues only for a sub-set of the original functions. As the dual-EF-containing species are distantly related to each other, we propose that independent re-modelling of EF-1α function took place in multiple branches in the tree of eukaryotes.

【 授权许可】

   
2013 Kamikawa et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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