First report of avocado tree wilt by Pythium sp. amazonianum in Mexico

Ciencia e Investigación Agraria

Juan Carlos Delgado¹  Mariana Beltran²  Omegar Hernandez³  Luis Mario Tapia⁴  Ernesto Cerna⁵  Yisa María Ochoa⁵  Anselmo Hernandez⁵ 

[1] CATEDRÁTICO CONACYT-UAAAN;CULTA S.A. DE C.V.;CULTA S.A. de C.V;Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Campo Experimental Uruapan;Universidad Autónoma Agraria Antonio Narro

关键词: Avocado;    Michoacan;    oomycete;    Pythium;    pathogenicity;   

DOI  :  10.7764/rcia.v45i3.1905

学科分类：农业科学（综合）

来源: Pontificia Universidad Catolica de Chile

【 摘 要 】

In August and September 2014, different avocado orchards ( Persea americanaMill. Var. Hass) were sampled in four municipalities (Tancítaro, Uruapan, San Juan Nuevo Parangaricutiro and Peribán) of the “Franja aguacatera” in Michoacan State, Mexico. Samples were collected from avocado trees showing symptoms of the “Tristeza del aguacate” disease. Strains of Pythium sp. were obtained consistently from symptomatic tissue isolated in the selective medium V8®-Agar-PARPH. Morphological identification was made through taxonomic keys, and molecular identification was obtained by sequencing Internal Transcribed Spacer (ITS) gene. Koch’s postulates were corroborated by pathogenicity tests carried out onP. americanaseedlings with six true leaves, identifying the strain ofP. sp. amazonianumas the causal agent of the “Tristeza del aguacate” disease. This is the first report that associates the “Tristeza del aguacate” withP. sp. amazonianumin Michoacan, Mexico.

【 授权许可】

Unknown