Identification and characterization of microRNAs in the ovaries of multiple and uniparous goats (Capra hircus) during follicular phase

BMC Genomics

Zi-Jun Zhang²  Xiao-Rong Zhang²  Yun-Hai Zhang²  Jian-Chuan Luo²  Ya-Feng Huang²  Li-Na Xu¹  Xiao-Fei Guo²  Chun-Huan Ren²  Ying-Hui Ling² 

[1] Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, No. 40 South Nongke Road, Hefei 230031, P.R. China;Anhui Provincial Laboratory of Local Animal Genetic Resources Conservation and Biobreeding, No. 130 Changjiang west road, Hefei 230036, P.R. China

关键词: Goat;    Follicular phase;    Ovary;    Solexa sequencing;    Kidding rate;    MicroRNA;   

Others  :  1217259
DOI  :  10.1186/1471-2164-15-339

 received in 2014-02-08, accepted in 2014-04-30,  发布年份 2014

【 摘 要 】

Background

Superior kidding rate is an important economic trait in production of meat goat, and ovulation rate is the precondition of kidding rate. MicroRNAs (miRNAs) play critical roles in almost all ovarian biological processes, including folliculogenesis, follicle development, follicle atresia, luteal development and regression. To find out the different ovarian activity and follicle recruitment with miRNA-mediated posttranscriptional regulation, the small RNAs expressed pattern in the ovarian tissues of multiple and uniparous Anhui White goats during follicular phase was analyzed using Solexa sequencing data.

Results

1008 miRNAs co-expressed, 309 and 433 miRNAs specifically expressed in the ovaries of multiple and uniparous goats during follicular phase were identified. The 10 most highly expressed miRNAs in the multiple library were also the highest expressed in the uniparous library, and there were no significantly different between each other. The highest specific expressed miRNA in the multiple library was miR-29c, and the one in the uniparous library was miR-6406. 35 novel miRNAs were predicted in total. GO annotation and KEGG Pathway analyses were implemented on target genes of all miRNA in two libraries. RT-PCR was applied to detect the expression level of 5 randomly selected miRNAs in multiple and uniparous hircine ovaries, and the results were consistent with the Solexa sequencing data.

Conclusions

In the present study, the different expression of miRNAs in the ovaries of multiple and uniparous goats during follicular phase were characterized and investigated using deep sequencing technology. The result will help to further understand the role of miRNAs in kidding rate regulation and also may help to identify miRNAs which could be potentially used to increase hircine ovulation rate and kidding rate in the future.

【 授权许可】

   
2014 Ling et al.; licensee BioMed Central Ltd.

【 图 表 】

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