A novel angiomatoid epithelioid sarcoma cell line, Asra-EPS, forming tumors with large cysts containing hemorrhagic fluid in vivo

BMC Research Notes

Hideki Yoshikawa⁴  Akira Myoui⁴  Eiichi Morii²  Ken-ichi Yoshida²  Mitsunori Kaya³  Ritsuro Ozaki⁴  Ken-ichiro Hamada⁴  Satoshi Takenaka⁴  Hirohiko Yasui⁴  Hidetatsu Outani⁴  Norifumi Naka¹  Yoshinori Imura⁴ 

[1] Department of Biology, Osaka Medical Center of Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka 537-8511, Japan;Department of Pathology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan;Department of Orthopaedic Surgery, Chitose City Hospital, 2-1-1 Hokko, Chitose, Hokkaido 066-8550, Japan;Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan

关键词: Cystogenesis;    INI-1;    CA 125;    VEGF;    Asra-EPS;    Epithelioid sarcoma;   

Others  :  1142005
DOI  :  10.1186/1756-0500-6-305

 received in 2013-04-23, accepted in 2013-07-29,  发布年份 2013

【 摘 要 】

Background

Whereas we can use several human epithelioid sarcoma (ES) cell lines for basic and preclinical research, an angiomatoid ES cell line has not been reported to date. We have treated a case of an angiomatoid ES developing in the right upper extremity of a 67-year-old man.

Methods

An angiomatoid ES cell line, Asra-EPS was newly established and characterized for its morphology, growth rate and chromosomal analysis. Tumorigenicity of Asra-EPS cells was also analyzed in athymic nude mice.

Results

Asra-EPS cells were round, polygonal or spindle-shaped with an abundant cytoplasm and have been maintained continuously in vitro for over 150 passages during more than 15 months. These cells secreted cancer antigen 125 (CA 125), interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) into the culture medium. Asra-EPS cells were tumorigenic when implanted in nude mice with tumors reaching a volume of 1000 mm³ at around 50 days. Histological features of tumors formed in mice were essentially the same as those of the original tumor, exhibiting a multinodular proliferation of eosinophilic epithelioid and spindle-shaped cells with prominent areas of hemorrhage and blood-filled cystic spaces strikingly corresponding to the potential of hemorrhagic cyst formation in the original tumor. They showed immunopositive staining for cytokeratins (AE1/AE3 and CAM5.2), epithelial membrane antigen (EMA), vimentin, CD31, CD34 and CA 125, but negative for integrase interactor 1 (INI-1) and factor VIII-related antigen.

Conclusions

The established cell line represents a biologically relevant new tool to investigate the molecular pathology of human angiomatoid ES and to evaluate the efficacy of novel therapeutics both in vitro and in vivo.

【 授权许可】

   
2013 Imura et al.; licensee BioMed Central Ltd.

【 图 表 】

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