Obesity in school-aged children and its correlation with Gut E.coli and Bifidobacteria: a case–control study

BMC Pediatrics

Chaomin Wan³  Ling Feng³  Linghan Kuang²  Liang Xie⁴  Ruizhen Jia¹  Xiaolin Gao³ 

[1]Open Laboratory, West China Institute for Women’s and Children’s Health, Chengdu, 610041, Sichuan, China

[2]Department of Laboratory Medicine, Sichuan University West China Second Hospital, Chengdu, 610041, Sichuan, China

[3]Department of Pediatrics, West China University Second Hospital, Sichuan University, Chengdu, 610041, Sichuan, China

[4]Pulmonary Vascular Remodeling Research Unit Laboratory, West China Institute for Women’s and Children’s Health, Chengdu, 610041, Sichuan, China

关键词: and Bifidobacteria;    E.coli;    Gut microbes;    Children;    Obesity;   

Others  :  1212105
DOI  :  10.1186/s12887-015-0384-x

 received in 2014-08-13, accepted in 2015-05-20,  发布年份 2015

【 摘 要 】

Background

To determine the correlation between obesity in school-aged children and imbalance of gut microbes by examining the ratio change of intestinal Bifidobacteria and E.coli in obese children compared to non-obese controls.

Methods

A hospital-based 1:1 case–control study was performed. Fecal samples of the subjects were collected for DNA extraction and analyzed by quantitative real-time PCR (qPCR) to determine the copy number of Bifidobacteria and E.coli. The ratio of two microbes (B/E) was then calculated and statistically analyzed.

Results

Subjects of the obesity group and control group showed no significant difference in age, gender or height (P > 0.05); whereas they had significant differences in body weight and BMI. Copy numbers of Bifidobacteria and E.coli per gram of wet fecal samples were first determined using qPCR in both obese and normal groups, which were further used for the calculation of B/E ratio. We found that B/E ration in the two groups showed significant difference (P < 0.05). Corrected χ² test was performed for the two groups against B/E < 1, and it was found that there was a positive correlation (OR = 719.2, OR 95% C.I. = 81.57-6341.18) between B/E ratio decrease with childhood obesity.

Conclusions

The obese children have a lower amount of Bifidobacteria and higher amount of E.coli (smaller B/E ratio) compared to normal non-obese children. It was suggested that obesity in children may be associated with the imbalance of gut microbes.

【 授权许可】

   
2015 Gao et al.; licensee BioMed Central.

【 图 表 】

Fig. 1.

【 参考文献 】

• [1]Robert MK, Bonita FS, Joseph WSG et al.. Overweight and obesity. Nelson textbook of pediatrics 19th. 2011.
• [2]Bulbul T, Hoque M. Prevalence of childhood obesity and overweight in Bangladesh: findings from a countrywide epidemiological study. BMC Pediatr. 2014; 14(1):86. BioMed Central Full Text
• [3]Pan L, May AL, Wethington H, Dalenius K, Grummer-Strawn LM. Incidence of obesity among young U.S. children living in low-income families, 2008–2011. Pediatrics. 2013; 132(6):1006-1013.
• [4]Ley RE, Turnbaugh PJ, Klein S, Gordon JI. Microbial ecology: human gut microbes associated with obesity. Nature. 2006; 444(7122):1022-1023.
• [5]Jumpertz R, Le DS, Turnbaugh PJ, Trinidad C, Bogardus C, Gordon JI et al.. Energy-balance studies reveal associations between gut microbes, caloric load, and nutrient absorption in humans. Am J Clin Nutr. 2011; 94(1):58-65.
• [6]Blasco-Baque V, Serino M, Burcelin R. Metabolic therapy at the edge between human hosts and gut microbes. Ann Pharm Fr. 2013; 71(1):34-41.
• [7]Santacruz A, Collado MC, Valdes G, Sequra MT, Martin-Lagos JA, Anjos T et al.. Gutmicrobiota composition isassociated withbody weight, weight gain andbiochemical parameters inpregnant women. Br J Nutr. 2010; 104(1):83-92.
• [8]The use and interpretation of anthropometry.Report of a WHO Expert Committee.Technical Report Series No.854. WHO, Geneva; 1995.
• [9]World health organization-Obesity and overweight-Fact sheet N°311-Updated March 2011; http://www.who.int/mediacentre/factsheets/fs311/en/.
• [10]Inukai T. Symptomatic obesity–classification, pathogenesis, diagnosis and therapy. Nihon Rinsho. 2013; 71(2):291-296.
• [11]Alan W, Julian P. Fighting obesity with bacteria. Science. 2013; 341:1069-1070.
• [12]Nieuwdorp M, Gilijamse PW, Pai N, Kaplan LM. Role of the microbiome in energy regulation and metabolism. Gastroenterology. 2014; 146(6):1525-1533.
• [13]Kallus SJ, Brandt LJ. The intestinal microbiota and obesity. J Clin Gastroenterol. 2012; 46(1):16-24.
• [14]Flint HJ. Obesity and the gut microbiota. J Clin Gastroenterol. 2011; 45:128-132.
• [15]Cho I, Yamanishi S, Cox L, Methe BA, Zavadil J, Li K et al.. Antibiotics in early life alter the murine colonic microbiome and adiposity. Nature. 2012; 488:621-626.
• [16]Koren O, Goodrich JK, Cullender TC, Spor A, Laitinen K, Backhed HK et al.. Host remodeling of the gut microbiome and metabolic changes during pregnancy. Cell. 2012; 150:470-480.
• [17]Million M, Raoult D. The role of the manipulation of the gut microbiota in obesity. Curr Infect Dis Rep. 2012; 15:25-30.
• [18]Million M, Angelakis E, Maraninchi M, Henry M, Giorgi R, Valero R et al.. Correlation betweenbody mass index andgutconcentrations oflactobacillus reuteri, bifidobacterium animalis, methanobrevibacter smithii andescherichia coli. Int J Obes (Lond). 2013; 37(11):1460-1466.
• [19]Iwai-Itamochi M, Yoshida H, Obara-Nagoya M, Horimoto E, Kurata T, Takizawa T. Development of real-time PCR to detect oral vaccine-like poliovirus and its application to environmental surveillance. J Virol Methods. 2014; 195:148-155.
• [20]Rodriguez-Lazaro D, Cook N, Hernandez M. Real-time PCR in food science: PCR diagnostics. Curr Issues Mol Biol. 2013; 15(2):39-44.
• [21]Van der Waaij D, Berghuis-de Vries JM, Lekkerkerk V. Colonization resistance of the digestive tract in conventional and antibiotic-treated mice. J Hyg(Lond). 1971; 69(3):405-411.
• [22]Lu H, Wu Z, Xu W, Yang J, ChEN Y, Li L. Intestinal microbiota was assessed in cirrhotic patients with hepatitis B virus infection. Intestinal microbiota of HBV cirrhotic patients. Microb Ecol. 2011; 61(3):693-703.