Longitudinal genetic analyses of Staphylococcus aureus nasal carriage dynamics in a diverse population

BMC Infectious Diseases

Alexander M Cole³  Christopher L Parkinson¹  Sergio Tafur⁴  Alana B Persaud³  Vanathy Paramanandam³  Austin Ellis³  Ryan P Lamers²  Gowrishankar Muthukrishnan³ 

[1]Department of Biology, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL, 32816, USA

[2]Current affiliation: Department of Biochemistry and Biomedical Sciences, Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada

[3]Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, 4000 Central Florida Boulevard, Orlando, FL, 32816, USA

[4]Stokes Advanced Research Computing Center, Institute for Simulation and Training, University of Central Florida, 3100 Technology Parkway, Orlando, FL, 32826, USA

关键词: SCCmec typing;    MRSA;    spa typing;    Nasal colonization;    Multi locus sequence typing;    Bacterial genetics;    Staphylococcus aureus;   

Others  :  1148394
DOI  :  10.1186/1471-2334-13-221

 received in 2012-12-10, accepted in 2013-05-08,  发布年份 2013

【 摘 要 】

Background

Staphylococcus aureus (SA) nasal colonization plays a critical role in the pathogenesis of staphylococcal infections and SA eradication from the nares has proven to be effective in reducing endogenous infections. To understand SA nasal colonization and its relation with consequent disease, assessment of nasal carriage dynamics and genotypic diversity among a diverse population is a necessity.

Results

We have performed extensive longitudinal monitoring of SA nasal carriage isolates in 109 healthy individuals over a period of up to three years. Longitudinal sampling revealed that 24% of the individuals were persistent SA nasal carriers while 32% were intermittent. To assess the genetic relatedness between different SA isolates within our cohort, multi locus sequence typing (MLST) was performed. MLST revealed that not only were strains colonizing intermittent and persistent nasal carriers genetically similar, belonging to the same clonal complexes, but strain changes within the same host were also observed over time for both types of carriers. More highly discriminating genetic analyses using the hypervariable regions of staphylococcal protein A and clumping factor B virulence genes revealed no preferential colonization of specific SA strains in persistent or intermittent carriers. Moreover, we observed that a subset of persistent and intermittent carriers retained clinically relevant community-acquired methicillin-resistant SA (CA-MRSA) strains in their nares over time.

Conclusions

The findings of this study provides added perspective on the nasal carriage dynamics between strains colonizing persistent and intermittent carriers; an area currently in need of assessment given that persistent carriers are at greater risk of autoinfection than intermittent carriers.

【 授权许可】

   
2013 Muthukrishnan et al.; licensee BioMed Central Ltd.

【 图 表 】

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