A method for quantifying pulmonary Legionella pneumophila infection in mouse lungs by flow cytometry

BMC Research Notes

Ian Richard van Driel²  Elizabeth Louise Hartland¹  Andrew Stephen Brown²  Sze Ying Ong¹  Desmond Koon Yong Ang² 

[1] Department of Microbiology and Immunology, The University of Melbourne, Parkville, VIC, 3010, Australia;Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, 3010, Australia

关键词: Mouse infection model;    Flow cytometry;    Neutrophil;    Legionnaires’ disease;    Legionella pneumophila;   

Others  :  1165917
DOI  :  10.1186/1756-0500-5-448

 received in 2012-05-30, accepted in 2012-08-15,  发布年份 2012

【 摘 要 】

Background

Pulmonary load of Legionella pneumophila in mice is normally determined by counting serial dilutions of bacterial colony forming units (CFU) on agar plates. This process is often tedious and time consuming. We describe a novel, rapid and versatile flow cytometric method that detects bacteria phagocytosed by neutrophils.

Findings

Mice were infected with L. pneumophila via intratracheal or intranasal administration. At various times after bacteria inoculation, mouse lungs were harvested and analysed concurrently for bacterial load by colony counting and flow cytometry analysis. The number of L. pneumophila-containing neutrophils correlated strongly with CFU obtained by bacteriological culture.

Conclusions

This technique can be utilised to determine pulmonary bacterial load and may be used in conjunction with other flow cytometric based analyses of the resulting immune response.

【 授权许可】

   
2012 Ang et al.; licensee BioMed Central Ltd.

【 图 表 】

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