Evaluation of antihyperglycemic and antinociceptive activity of Xanthium indicum stem extract in Swiss albino mice

BMC Complementary and Alternative Medicine

Rownak Jahan¹  Mohammed Rahmatullah²  Shahnaz Rahman¹  Md Ezazul Haque¹ 

[1] Department of Biotechnology and Genetic Engineering, University of Development Alternative, Dhanmondi, Dhaka 1209, Bangladesh;Faculty of Life Sciences, University of Development Alternative, House No. 78, Road No. 11A (new), Dhanmondi, Dhaka 1209, Bangladesh

关键词: Asteraceae;    Antinociceptive;    Glucose tolerance;    Xanthium indicum;    Antihyperglycemic;   

Others  :  1220835
DOI  :  10.1186/1472-6882-13-296

 received in 2013-08-15, accepted in 2013-10-29,  发布年份 2013

【 摘 要 】

Background

Xanthium indicum stem is used in folk medicine of Bangladesh to control sugar in diabetic patients and to alleviate pain. The objective of the study was to evaluate antihyperglycemic and antinociceptive activity of methanolic extract of Xanthium indicum stems (XISE) in mice.

Methods

Antihyperglycemic activity was measured by oral glucose tolerance tests in glucose-loaded Swiss albino mice. Antinociceptive activity was determined by observed decreases in abdominal constrictions in acetic acid-induced gastric pain model in mice.

Results

The methanol extract of stems showed dose-dependent and statistically significant antihyperglycemic activity at doses of 50, 100, 200 and 400 mg per kg body weight (p values, respectively, < than 0.01, 0.01, 0.005, and 0.01). Highest reduction in blood glucose level (31.2%) was observed with the highest dose (400 mg) of the extract. A standard antihyperglycemic drug, glibenclamide, reduced blood glucose levels by 46.2%, when administered at a dose of 10 mg per kg body weight. In antinociceptive activity tests, the extract when administered at the afore-mentioned four doses, reduced the number of abdominal constrictions in mice, respectively, by 41.7, 50.0, 54.2, and 61.0%. In comparison, a standard antinociceptive drug, aspirin, when administered at a dose of 200 mg per kg body weight, reduced the number of abdominal constrictions by 37.5%.

Conclusion

The experimental results obtained in the present study validate the use of X. indicum stems in folk medicines of Bangladesh to lower blood sugar in diabetic patients and to alleviate pain.

【 授权许可】

   
2013 Haque et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Rahmatullah M, Ishika T, Rahman M, Swarna A, Khan T, Monalisa MN, Seraj S, Mou SM, Mahal MJ, Biswas KR: Plants prescribed for both preventive and therapeutic purposes by the traditional healers of the Bede community residing by the Turag River, Dhaka District. Am-Eur J Sustain Agric 2011, 5:325-331.
• [2]Namsa ND, Tag H, Mandal M, Kalita P, Das AK: An ethnobotanical study of traditional anti-inflammatory plants used by the Lohit community of Arunachal Pradesh, India. J Ethnopharmacol 2009, 125:234-245.
• [3]Ullah MO, Haque M, Urmi KF, Zulfiker AH, Anita ES, Begum M, Hamid K, Uddin SJ: Anti-bacterial activity and brine shrimp lethality bioassay of methanolic extracts of fourteen different edible vegetables from Bangladesh. Asian Pac J Trop Biomed 2013, 3:1-7.
• [4]Gilani AH, Rahman AU: Trends in ethnopharmacology. J Ethnopharmacol 2005, 100:43-49.
• [5]Joy KL, Kuttan RJ: Anti-diabetic activity of Picrorrhiza kurroa extract. J Ethnopharmacol 1999, 67:143-148.
• [6]Nyunai N, Njikam N, Addennebi EH, Mbaford JT, Lamnaouer D: Hypoglycaemic and antihyperglycaemic activity of Ageratum conyzoides L. in rats. Afr J Tradit Complement Altern Med 2009, 6:123-130.
• [7]Shanmugasundaram P, Venkataraman S: Anti-nociceptive activity of Hygrophilous auriculata (Schum) Heine. Afr J Tradit Complement Altern Med 2005, 2:62-69.
• [8]Khatun F, Zaman F, Mosaiab T, Mostafa F, Zaman M, Rehana F, Nasrin D, Jamal F, Nahar N, Rahmatullah M: Evaluation of antinociceptive and antihyperglycemic activities in methanol extracts of whole plants of Alternanthera philoxeroides (Mart.) Griseb. (Amaranthaceae) in mice. Pak J Pharm Sci 2012, 25:583-587.
• [9]Venkatesh S, Reddy GD, Reddy YSR, Sathyavathy D, Reddy BM: Effect of Helicteres isora root extracts on glucose tolerance in glucose-induced hyperglycemic rats. Fitoterapia 2004, 75:364-367.
• [10]Sulaiman MR, Hussain MK, Zakaria ZA, Somchit MN, Moin S, Mohamad AS, Israf DA: Evaluation of the antinociceptive activity of Ficus deltoidea aqueous extract. Fitoterapia 2008, 2008(79):557-561.
• [11]Rang HP, Dale MM, Ritter JM, Moore PK: Pharmacology. 5th edition. Edinburgh: Churchill Livingstone; 2003:562-563.
• [12]Reynolds JEF: Martindale: The Extra Pharmacopoeia. 28th edition. London: The Pharmaceutical Press; 1982:245.
• [13]Hossan ANMF, Zaman F, Barman MR, Khatoon S, Zaman M, Khatun F, Mosaiab T, Mostafa F, Sintaha M, Jamal F, Rahmatullah M: Antinociceptive activity of Xanthium indicum J. Koenig ex Roxb. (Asteraceae) leaves and Leucas aspera (Willd.) Link (Lamiaceae) whole plants. Adv Nat Appl Sci 2011, 5:214-217.
• [14]Hasan SMR, Akter R, Hossain MM, Faruque A, Rana MS: Antinociceptive and CNS depressant activities of Xanthium indicum Koen. in mice. Dhaka Univ J Pharm Sci 2009, 8:99-101.
• [15]Kupiecki FP, Ogzewalla CD, Schell FM: Isolation and characterization of a hypoglycemic agent from Xanthium strumarium. J Pharm Sci 1974, 63:1166-1167.
• [16]Han T, Li HL, Zhang QY, Han P, Zheng HC, Rahman K, Qin LP: Bioactivity-guided fractionation for anti-inflammatory and analgesic properties and constituents of Xanthium strumarium L. Phytomedicine 2007, 14:825-829.