In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell

BMC Complementary and Alternative Medicine

Cheng Lian Gwendoline-Ee³  Fauziah Othman²  Noorjahan Banu Alitheen⁴  Abdah Md Akim²  Swee Keong Yeap⁴  Asmah Rahmat¹  Hueh Zan Chong⁵ 

[1] Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia;Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia;Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia;Department of Cell and Molecule Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia;School of Pharmacy and Health Sciences, International Medical University, No. 126, Jalan Jalil Perkasa 19, Bukit Jalil Kuala Lumpur, 57000 Malaysia

关键词: p53;    Apoptosis;    MCF-7;    Strobilanthes crispus;   

Others  :  1232720
DOI  :  10.1186/1472-6882-12-35

 received in 2012-01-11, accepted in 2012-04-04,  发布年份 2012

【 摘 要 】

Background

Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear.

Results

Strobilanthes cripus was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC₅₀ of S. crispus ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study.

Conclusion

Our findings suggest that S. crispus ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway.

【 授权许可】

   
2012 Chong et al; licensee BioMed Central Ltd.

【 图 表 】

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