Expression of SHH signaling molecules in the developing human primary dentition

BMC Developmental Biology

Yanding Zhang¹  YiPing Chen²  Zhen Huang¹  Chensheng Lin¹  Guimiao Chen¹  Shuo Zhang¹  Xuefeng Hu¹ 

[1] Fujian Key Laboratory of Developmental and Neuro Biology, College of Life Science, Fujian Normal University, Fuzhou, Fujian, 350108, P.R. China;Department of Cell and Molecular Biology, Tulane University, New Orleans, LA, 70118, USA

关键词: GLI;    SMO;    PTC;    SHH;    Gene expression;    Tooth development;    Deciduous tooth;   

Others  :  1085962
DOI  :  10.1186/1471-213X-13-11

 received in 2012-10-04, accepted in 2013-04-04,  发布年份 2013

【 摘 要 】

Background

Our current knowledge on tooth development derives primarily from studies in mice. Very little is known about gene expression and function during human odontogenesis. Sonic Hedgehog (SHH) signaling has been demonstrated to play crucial roles in the development of multiple organs in mice, including the tooth. However, if SHH signaling molecules are expressed and function in the developing human embryonic tooth remain unknown.

Results

We conducted microarray assay to reveal the expression profile of SHH signaling pathway molecules. We then used in situ hybridization to validate and reveal spatial and temporal expression patterns of a number of selected molecules, including SHH, PTC1, SMO, GLI1, GLI2, and GLI3, in the developing human embryonic tooth germs, and compared them with that in mice. We found that all these genes exhibit similar but slightly distinct expression patterns in the human and mouse tooth germ at the cap and bell stages.

Conclusions

Our results demonstrate the operation of active SHH signaling in the developing human tooth and suggest a conserved function of SHH signaling pathway during human odontogenesis.

【 授权许可】

   
2013 Hu et al.; licensee BioMed Central Ltd.

【 图 表 】

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