Fungal keratitis caused by Beauveria bassiana: drug and temperature sensitivity profiles: a case report

BMC Research Notes

Yuichi Ohashi²  Yuko Hara⁵  Yasuaki Yamamoto⁵  Xiaodong Zheng⁵  Seishi Asari⁴  Akiko Ueda⁴  Atsuko Sunada⁴  Hitoshi Miyamoto³  Atsushi Shiraishi¹  Arisa Mitani⁵ 

[1] Department of Stem cell Biology, Shitsukawa, Toon, Ehime 791-0295, Japan;Department of Infectious Diseases Medicine, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime 791-0295, Japan;Department of Clinical Laboratory, Ehime University Hospital, Shitsukawa, Toon, Ehime 791-0295, Japan;Department of Clinical Laboratory, Osaka University Hospital, Suita, Osaka Prefecture 565-0871, Japan;Department of Ophthalmology, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime 791-0295, Japan

关键词: Temperature sensitivity growth;    Drug-sensitivity;    In vivo confocal microscopy;    Beauveria bassiana;    Fungal keratitis;   

Others  :  1127398
DOI  :  10.1186/1756-0500-7-677

 received in 2014-04-29, accepted in 2014-09-22,  发布年份 2014

【 摘 要 】

Background

Beauveria bassiana is an entomopathogenic fungus and is a rare cause of keratitis. We present a case of fungal keratitis caused by B. bassiana that was diagnosed by in vivo confocal microscopy and in vitro corneal cultures. In addition, we determined the temperature- and drug-sensitivities of the isolated strain of B. bassiana.

Case presentation

A 59-year-old Japanese man with a 2-month history of keratitis was examined by slit-lamp biomicroscopy, in vivo confocal microscopy, and histology and cultures of corneal scrapings. The corneal scrapings were used to determine the minimal inhibitory concentrations of different antifungal drugs and also to determine the temperature-sensitivity. In vivo confocal microscopy and histological examinations showed filamentous fungal keratitis. The characteristics of the fungal growth indicated that the keratitis was caused by B. bassiana. The keratitis responded poorly to systemic and topical voriconazole and to natamycin ointment. However, it was resolved after changing the natamycin to micafungin combined with surgical debridement. The isolated strain was sensitive to itraconazole, miconazole, micafungin, voriconazole, and resistant to flucytosine and fluconazole. It was moderately sensitive to amphotericin B, and natamycin. After 7 days in culture, the isolate grew small white colonies at 25°C, very small colonies at 35°C and 37°C.

Conclusion

The drug-sensitivity and temperature-sensitivity profiles of B. bassiana should be helpful in the treatment of B. bassiana keratitis. Therapeutic surgery may be helpful for mycotic keratitis poorly responsive to medical therapy alone.

【 授权许可】

   
2014 Mitani et al.; licensee BioMed Central Ltd.

【 图 表 】

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