Invasion of Ureaplasma diversum in bovine spermatozoids

BMC Research Notes

Jorge Timenetsky¹  Mayra EO Assumpção¹  Gláucia M Machado-Santelli¹  Lucas Miranda Marques¹  Beatriz A Cortez¹  Rosângela C Oliveira¹  Maurício Yamaguti¹  Melissa Buzinhani¹ 

[1] Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brasil

关键词: confocal microscopy;    invasion;    bovine spermatozoid;    Ureaplasma diversum;   

Others  :  1167015
DOI  :  10.1186/1756-0500-4-455

 received in 2011-05-18, accepted in 2011-10-27,  发布年份 2011

【 摘 要 】

Background

Ureaplasma diversum has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze in vitro interaction of U. diversum isolates and ATCC strains with bovine spermatozoids. The interactions were observed by confocal microscopy and the gentamycin internalization assay.

Findings

U. diversum were able to adhere to and invade spermatozoids after 30 min of infection. The gentamicin resistance assay confirmed the intracellularity and survival of U. diversum in bovine spermatozoids.

Conclusions

The intracellular nature of bovine ureaplasma identifies a new difficulty to control the reproductive of these animals.

【 授权许可】

   
2010 Timenetsky et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

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