Multiple human papillomavirus infections are highly prevalent in the anal canal of human immunodeficiency virus-positive men who have sex with men

BMC Infectious Diseases

Alejandro García-Carrancá²  David Cantú de León⁴  Silvia C Galván¹  Yanink Caro-Vega³  Juan G Sierra-Madero³  Brenda Crabtree-Ramírez³  Norma E Rivera-Martínez³  Rocío Méndez-Martínez⁵ 

[1] Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México D.F., Mexico;Laboratory of Virus & Cancer Instituto Nacional de Cancerología, Av. San Fernando No. 22, Colonia Sección XVI, México D.F. 14080, Tlalpan, México;Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán,” SSA, México D.F., Mexico;Dirección de Investigación Clínica, Instituto Nacional de Cancerología, Secretaría de Salud (SSA), México D.F., Mexico;División de Investigación, Instituto Nacional de Cancerología, Secretaría de Salud (SSA), México D.F., Mexico

关键词: Anal cancer;    Variants;    Type 16;    MSM;    HPV;    HIV;   

Others  :  1090817
DOI  :  10.1186/s12879-014-0671-4

 received in 2013-12-04, accepted in 2014-12-01,  发布年份 2014

【 摘 要 】

Background

Anal cancer has become one of the most common non-AIDS-defined tumors among Human Immunodeficiency Virus-positive (HIV+) individuals, and a rise in its incidence among HIV+ Men who have Sex with Men (MSM) has been shown, despite the introduction of Highly Active Anti-Retroviral Therapy (HAART). Human Papillomavirus (HPV) infections are highly prevalent among HIV+ MSM and recent studies have shown high rates of HPV-associated anal intraepithelial neoplasia (AIN) and anal cancer among this population.

Methods

In the present study we determined the prevalence and nature of HPV co-infections in the anal canal of 324 HIV+ MSM attending a high specialty medical center in Mexico City, DNA extraction and amplification with generic primers for HPV was performed, followed by detection of specific types and co-infections with INNO-Lipa, and identification of variants by amplification and sequencing of the E6 and LCR region of HPV 16.

Results

We found a very high prevalence of HPV infections among this cohort (86%), with more than one fourth of them (28%) positive for type 16. Among HPV16-positive patients, European variants were the most prevalent, followed by Asian-American ones. Among these individuals (HPV-16+), we identified co-infections with other 21 HPV types namely; 11, 51, 52, 6, 66, 68, 74, 18, 45, 35, 26, 44, 70, 53, 54, 82, 31, 33, 56, 58, 59.

Conclusions

HIV+ MSM show a very high rate of HPV infections in the anal canal and those with type 16 exhibited a multiplicity of associated types. This study emphasizes the need for an early detection of HPV infections among HIV+ MSM in order to establish its utility to prevent anal neoplasia and cancer.

【 授权许可】

   
2014 Méndez Martínez et al.; licensee BioMed Central Ltd.

【 图 表 】

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