Cultivation conditions and the diffusion of oxygen into culture media: The rationale for the flask-to-medium ratio in microbiology

BMC Microbiology

Richard A Proctor¹  Greg A Somerville² 

[1] Departments of Medicine and Medical Microbiology/Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA;School of Veterinary Medicine and Biomedical Sciences, University of Nebraska, Lincoln, NE 68583-0905, USA

关键词: Oxygen diffusion;    Aeration;    Cultivation conditions;   

Others  :  1144605
DOI  :  10.1186/1471-2180-13-9

 received in 2013-01-03, accepted in 2013-01-14,  发布年份 2013

【 摘 要 】

Bacterial cultivation requires consideration of three things: The bacterial strain, cultivation medium, and cultivation conditions. Most microbiologists dutifully report their choice of strains and cultivation media in manuscripts; however, these same microbiologists often overlook reporting cultivation conditions. Without this information, it is difficult to determine if cultures were grown aerobically, microaerobically, or anaerobically. To cultivate bacteria aerobically, it is necessary to understand that oxygen does not readily diffuse into culture media; it needs help to get in. Microbiologists can do this by altering the flask-to-medium ratio, rpm of agitation, and/or the concentration of atmospheric oxygen, or by using baffled flasks.

【 授权许可】

   
2013 Somerville and Proctor; licensee BioMed Central Ltd.

【 图 表 】

Fig. 9.

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