Neuroglobin-overexpression reduces traumatic brain lesion size in mice

BMC Neuroscience

Xiaoying Wang⁴  Eng H Lo³  Josephine M Lok¹  Michael J Whalen¹  Kazuhide Hayakawa³  Changhong Xing³  Gang Zhao²  Zhanyang Yu³  Song Zhao³ 

[1]Department of Pediatrics, Pediatric Critical Care Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA

[2]Departments of Orthopedic and Neurosurgery, The First Bethune Hospital of Jilin University, Changchun, Jilin, China

[3]Neuroprotection Research Laboratory, Departments of Neurology and Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA

[4]Neuroprotection Research Laboratory, Departments of Neurology and Radiology, Massachusetts General Hospital, 149 13th Street, Room 2401, Charlestown, MA, 02129, USA

关键词: Traumatic brain injury;    Oxidative stress;    Controlled cortical impact;    Neuroprotection;    Neuroglobin;   

Others  :  1170670
DOI  :  10.1186/1471-2202-13-67

 received in 2012-03-21, accepted in 2012-05-24,  发布年份 2012

【 摘 要 】

Background

Accumulating evidence has demonstrated that over-expression of Neuroglobin (Ngb) is neuroprotective against hypoxic/ischemic brain injuries. In this study we tested the neuroprotective effects of Ngb over-expression against traumatic brain injury (TBI) in mice.

Results

Both Ngb over-expression transgenic (Ngb-Tg) and wild-type (WT) control mice were subjected to TBI induced by a controlled cortical impact (CCI) device. TBI significantly increased Ngb expression in the brains of both WT and Ngb-Tg mice, but Ngb-Tg mice had significantly higher Ngb protein levels at the pre-injury baseline and post-TBI. Production of oxidative tissue damage biomarker 3NT in the brain was significantly reduced in Ngb-Tg mice compared to WT controls at 6 hours after TBI. The traumatic brain lesion volume was significantly reduced in Ngb Tg mice compared to WT mice at 3 weeks after TBI; however, there were no significant differences in the recovery of sensorimotor and spatial memory functional deficits between Ngb-Tg and WT control mice for up to 3 weeks after TBI.

Conclusion

Ngb over-expression reduced traumatic lesion volume, which might partially be achieved by decreasing oxidative stress.

【 授权许可】

   
2012 Zhao et al.; licensee BioMed Central Ltd.

【 图 表 】

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