Illumina identification of RsrA, a conserved C2H2 transcription factor coordinating the NapA mediated oxidative stress signaling pathway in Aspergillus

BMC Genomics

Nancy P Keller³  Jennifer Wortman²  Brian Haas²  Fang Yun Lim³  Graeme S Garvey¹  Philipp Wiemann³  Jin Woo Bok³ 

[1] Current address: Monsanto Vegetable Seeds, 37437 State Highway 16, Woodland, CA 95695, USA;Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge MA, 02142, USA;Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI, USA

关键词: CCAAT;    AP-1;    Stress response;    Mutagenesis;    Next generation sequencing;    Aspergillus;   

Others  :  1091466
DOI  :  10.1186/1471-2164-15-1011

 received in 2014-11-04, accepted in 2014-11-12,  发布年份 2014

【 摘 要 】

Background

Chemical mutagenesis screens are useful to identify mutants involved in biological processes of interest. Identifying the mutation from such screens, however, often fails when using methodologies involving transformation of the mutant to wild type phenotype with DNA libraries.

Results

Here we analyzed Illumina sequence of a chemically derived mutant of Aspergillus nidulans and identified a gene encoding a C2H2 transcription factor termed RsrA for regulator of stress response. RsrA is conserved in filamentous fungal genomes, and upon deleting the gene in three Aspergillus species (A. nidulans, A. flavus and A. fumigatus), we found two conserved phenotypes: enhanced resistance to oxidative stress and reduction in sporulation processes. For all species, rsrA deletion mutants were more resistant to hydrogen peroxide treatment. In depth examination of this latter characteristic in A. nidulans showed that upon exposure to hydrogen peroxide, RsrA loss resulted in global up-regulation of several components of the oxidative stress metabolome including the expression of napA and atfA, the two bZIP transcription factors mediating resistance to reactive oxygen species (ROS) as well as NapA targets in thioredoxin and glutathione systems. Coupling transcriptional data with examination of ΔrsrAΔatfA and ΔrsrAΔnapA double mutants indicate that RsrA primarily operates through NapA-mediated stress response pathways. A model of RsrA regulation of ROS response in Aspergillus is presented.

Conclusion

RsrA, found in a highly syntenic region in Aspergillus genomes, coordinates a NapA mediated oxidative response in Aspergillus fungi.

【 授权许可】

   
2014 Bok et al.; licensee BioMed Central Ltd.

【 图 表 】

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