Coagulation activation, depletion of platelet granules and endothelial integrity in case of uraemia and haemodialysis treatment

BMC Nephrology

Piet CM Bartels²  Menso J Nubé¹  Margreet Schoorl²  Marianne Schoorl² 

[1]Department of Nephrology, VU Medical Centre, Amsterdam, The Netherlands

[2]Department of Clinical Chemistry, Haematology & Immunology, Medical Center Alkmaar, Alkmaar, The Netherlands

关键词: Renal failure;    End-stage kidney disease;    Proendothelin;    Coagulation;    Platelet granules depletion;   

Others  :  1082967
DOI  :  10.1186/1471-2369-14-72

 received in 2012-11-01, accepted in 2013-03-15,  发布年份 2013

【 摘 要 】

Background

During haemodialysis (HD) treatment, increase of platelet (PLT) activation and induction of procoagulant activity is demonstrated. Although the role of the endothelium and its direct interaction with coagulation and homeostasis is known, it is not elucidated how PLT activation markers and activation of coagulation coincide with markers of endothelial integrity during HD treatment. In the present study uraemia and HD induced changes, with particular emphasis on PLT granules depletion, activation of coagulation and endothelial integrity were investigated.

Methods

To detect depletion of PLT granules, peripheral blood slide smears were screened by light microscopy for qualitative evaluation of PLT granule containing cytoplasm, as indicated by its granules staining density. Activation of coagulation was investigated by establishement of thrombin-antithrombin (TAT) and fibrinogen concentrations. To evaluate endothelial integrity proendothelin (proET-1) plasma concentrations were established.

Results

Results of our study demonstrate that proET-1 plasma concentrations were obviously increased in the subjects’ group with end-stage chronic kidney disease (CKD) and renal failure if compared with a group of apparently healthy subjects. The amount of depleted PLT granules was obviously increased in the subjects’ group with end-stage CKD if compared with the group with renal failure. Mean plasma concentrations of TAT and fibrinogen revealed results within the reference range.

Conclusions

It is demonstrated that uraemia is associated with endothelial damage and aberrations in PLT granules morphology in subjects with HD treatment. We hypothesize that increased proET-1 concentrations reflect ongoing stress on endothelial cells amongst others due to uraemia. Biomarkers like proET-1 and aberrations in PLT granules morphology assist in the early detection of procoagulant activity of the endothelium.

【 授权许可】

   
2013 Schoorl et al.; licensee BioMed Central Ltd.

【 图 表 】

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