Molecular mechanism of apoptosis induction in skin cancer cells by the centipedegrass extract

BMC Complementary and Alternative Medicine

Byung Yeoup Chung²  Bo Yun Choi²  Dong Min Jang¹  Chul-Hong Park¹  Hyoung-Woo Bai²  Srilatha Badaboina² 

[1] School of Biological Sciences and Biotechnology, Chonnam National University, Gwangju 500-757, Republic of Korea;Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), Jeongeup-si, Jeollabuk-do 580-185, Republic of Korea

关键词: Skin cancer;    Caspase;    PI3K/AKT/GSK-3β;    SKMEL-5;    B16F1;    Apoptosis;    Centipedegrass extract;   

Others  :  1220456
DOI  :  10.1186/1472-6882-13-350

 received in 2013-03-15, accepted in 2013-11-29,  发布年份 2013

【 摘 要 】

Background

Centipedegrass extract (CGE) is mainly composed of maysin and its derivatives, which are recognized internationally as natural compounds. Compared to other flavonoids, maysin has a unique structure in that mannose is bound to the flavonoid backbone. CGE exhibits some biological properties in that it can function as an anti-oxidant, anti-inflammatory, anti-adipogenic, and insecticidal. Whether CGE has other biological functions, such as anti-cancer activity, is unknown.

Methods

B16F1 (mouse) and SKMEL-5 (human) cells were treated with CGE, and their subsequent survival was determined using MTT assay. We performed a cell cycle analysis using propidium iodide (PI), and detected apoptosis using double staining with annexin V-FITC/PI. In addition, we examined mitochondrial membrane potentials using flow cytometry, as well as signaling mechanisms with an immunoblotting analysis.

Results

CGE inhibited skin cancer cell growth by arresting the cell cycle in the G₂/M phase, and increased both early and late apoptotic cell populations without affecting normal cells. Furthermore, we observed mitochondrial transmembrane depolarization, increased cytochrome-c release, caspase-3 and caspase-7 activation, and increased poly ADP-ribose polymerase degradation. CGE also downregulated activation of p-AKT, p-glycogen synthase kinase-3β (GSK-3β), and p-BAD in a time-dependent manner. LY294002 inhibition of phosphoinositide 3-kinase (PI3K) significantly sensitized skin cancer cells, which led to an increase in CGE-induced apoptosis.

Conclusions

CGE controlled skin cancer cell growth by inhibiting the PI3K/AKT/GSK-3β signaling pathway and activating the effector caspases. This study is the first to demonstrate anti-cancer properties for CGE, and that CGE may be an effective therapeutic agent for treating skin cancer.

【 授权许可】

   
2013 Badaboina et al.; licensee BioMed Central Ltd.

【 图 表 】

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