High levels of multiresistance in quinolone resistant urinary tract isolates of Escherichia coli from Norway; a non clonal phenomen?

BMC Research Notes

Bjørn Erik Kristiansen³  Nils Grude¹  Anne Gry Allum⁴  Ingrid Høgli Henriksen²  Andrew Jenkins²  Linda Strand⁴ 

[1] Present address: Department of Medical Microbiology, Vestfold Trust Hospital, Tønsberg, Norway;Department of Environmental and Health Sciences, Telemark University College, Bø, Norway;Department of Microbiology and Virology, University of Tromsø, Tromsø, Norway;Present address: Telemark Hospital, Medical and Environmental Genetics Unit, Skien, Norway

关键词: PFGE;    Nalidixic acid;    Ciprofloxacin;    CGA;    Phylogenetic group;    Clone;    E. coli;    Multiresistance;   

Others  :  1132452
DOI  :  10.1186/1756-0500-7-376

 received in 2014-01-26, accepted in 2014-05-28,  发布年份 2014

【 摘 要 】

Background

The problem of emerging ciprofloxacin resistance is compounded by its frequent association with multiresistance, the reason for which is not fully understood. In this study we compare multiresistance, clonal similarities and phylogenetic group in urinary tract isolates of Escherichia coli sensitive and resistant to the quinolone antimicrobials nalidixic acid and ciprofloxacin.

Results

Quinolone resistant isolates were more resistant to non-quinolone antibiotics than sensitive isolates, with resistance to ampicillin, mecillinam, sulphonamide, trimethoprim, tetracycline, kanamycin and chloramphenicol significantly increased. Fifty-one percent of quinolone-resistant isolates were multiresistant. Although multiresistance was most prevalent (63%) in isolates showing high-level ciprofloxacin resistance, it was still highly prevalent (41%) in nalidixic acid resistant isolates with low-level ciprofloxacin resistance. Multiresistance was more frequent among singleton isolates (61%) than clonal isolates (40%) of quinolone resistant Escherichia coli. Ciprofloxacin resistance was associated with certain specific clones, among them the globally distributed clonal Group A. However, there was no significant difference in the overall degree of clonality between quinolone sensitive and resistant isolates. Ciprofloxacin resistance was positively associated with phylogroup D and negatively associated with phylogroup B2. This correlation was not associated with clonal isolates.

Conclusion

This study supports earlier findings of association between ciprofloxacin resistance and resistance to other antibiotics. The prevalence of multiresistance in quinolone-resistant isolates that have not yet developed high-level ciprofloxacin resistance suggest that multiresistance arises early in the development of quinolone resistance. This is consistent with exposure to quinolones causing quinolone resistance by mutations and mobilization of multiresistance elements by induction of the SOS response. The spread of clones seems to be less important than previously reported in regard to emergence of quinolone resistance and multiresistance as both are associated primarily with singleton isolates.

【 授权许可】

   
2014 Strand et al.; licensee BioMed Central Ltd.

【 图 表 】

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