RAGE controls leukocyte adhesion in preterm and term infants

BMC Immunology

David Frommhold¹  Johannes Poeschl¹  Hannes Hudalla¹  Navina Kuss¹  Natascha Braach¹  Marie-Sophie Metzger¹  Raphaela Tschada¹  Kirsten Buschmann¹ 

[1] Department of Neonatology, University Hospital, Heidelberg, 69120, Germany

关键词: Inflammation;    RAGE;    Leukocyte adhesion;    Neutrophils;    Neonate;    Preterm;    Fetal;   

Others  :  1089859
DOI  :  10.1186/s12865-014-0053-0

 received in 2014-07-29, accepted in 2014-11-03,  发布年份 2014

【 摘 要 】

Background

Insufficient leukocyte recruitment may be one reason for the high incidence of life-threatening infections in preterm infants. Since the receptor of advanced glycation end products (RAGE) is a known leukocyte adhesion molecule and highly expressed during early development, we asked whether RAGE plays a role for leukocyte recruitment in preterm and term infants.

Methods

Leukocyte adhesion was analyzed in dynamic flow chamber experiments using isolated leukocytes of cord blood from extremely premature (<30 weeks of gestation), moderately premature (30–35 weeks of gestation) and mature neonates (>35 weeks of gestation) and compared to the results of adults. For fluorescent microscopy leukocytes were labeled with rhodamine 6G. In the respective age groups we also measured the plasma concentration of soluble RAGE (sRAGE) by ELISA and Mac-1 and LFA-1 expression on neutrophils by flow cytometry.

Results

The adhesive functions of fetal leukocytes significantly increase with gestational age. In all age groups, leukocyte adhesion was crucially dependent on RAGE. In particular, RAGE was equally effective to mediate leukocyte adhesion when compared to ICAM-1. The plasma levels of sRAGE were high in extremely premature infants and decreased with increasing gestational age. In contrast, expression of β₂-Integrins Mac-1 and LFA-1 which are known ligands for RAGE and ICAM-1 did not change during fetal development.

Conclusion

We conclude that RAGE is a crucial leukocyte adhesion molecule in both preterm and term infants.

【 授权许可】

   
2014 Buschmann et al.; licensee BioMed Central Ltd.

【 图 表 】

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