High-throughput sequencing for the detection of the bacterial and fungal diversity in Mongolian naturally fermented cow’s milk in Russia

BMC Microbiology

Heping Zhang¹  Bilige Menhe²  Qiangchuan Hou²  Zhuang Guo²  Jiachao Zhang²  Zhihong Sun¹  Lai-Yu Kwok²  Yi Zheng²  Wenjun Liu¹ 

[1] Synergetic Innovation Center of Food Safety and Nutrition, Northeast Agricultural University, Harbin 150030, P. R. China;Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, P. R. China

关键词: Mongolia;    Chita;    Kalmykia;    Russia;    Naturally fermented cow’s milk;    Pyrosequencing;   

Others  :  1137363
DOI  :  10.1186/s12866-015-0385-9

 received in 2015-01-25, accepted in 2015-02-12,  发布年份 2015

【 摘 要 】

Background

Traditional fermented dairy products are major components of the typical Mongolian diet since ancient times. However, almost all the previous studies on the microbial composition of traditional Mongolian fermented dairy products analyzed food samples from the Chinese Mongolian region and Mongolia but not the Russian Mongolian region. In this study, the bacterial and fungal community diversity of nineteen naturally fermented cow’s milk (NFCM) samples from local Mongolian families residing in Kalmykia and Chita of Russia was investigated with pyrosequencing.

Results

Firmicutes and Ascomycota were the predominant phyla respectively for bacteria and fungi. The abundance of the bacterial phylum Acidobacteria was considerably different between the samples from the two regions. At genus level, Lactobacillus and Pichia were the predominating bacterial and fungal genera, respectively, while six bacterial genera significantly differed between the Kalmykia (enrichment of Aeromonas, Bacillus, Clostridium, Streptococcus, Vogesella) and Chita (enrichment of Lactococcus) samples. The results of principal coordinate analysis (PCoA) based on the bacterial or fungal composition of the Kalmykia and Chita samples revealed a different microbiota structure between the samples collected in these two locations. The redundancy analysis (RDA) identified 60 bacterial and 21 fungal OTUs as the key variables responsible for such microbiota structural difference.

Conclusions

Our results suggest that structural differences existed in the microbiota of NFCM between Kalmykia and Chita. The difference in geographic environment may be an important factor influencing the microbial diversity of NFCM made by the Mongolians in Russia.

【 授权许可】

   
2015 Liu et al.; licensee BioMed Central.

【 图 表 】

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