期刊论文详细信息
BMC Complementary and Alternative Medicine
Biochemical and haematological assessment of toxic effects of the leaf ethanol extract of Petroselinum crispum (Mill) Nyman ex A.W. Hill (Parsley) in rats
S Olatunbosun Banjoko1  Emmanuel Olorunju Awe2 
[1] Institute of Public Health, College of Health Sciences, Obafemi Awolowo University, Ile-Ife, Nigeria;Department of Pharmacology & Therapeutics, College of Health Sciences, Ladoke,Akintola University of Technology, Osogbo campus, P.M.B 4000, Ogbomoso, Nigeria
关键词: Rats;    Histopathology;    Haematology and biochemistry;    Toxicity;    Petroselinum crispum (Parsley);    Ethanol extract;   
Others  :  1230137
DOI  :  10.1186/1472-6882-13-75
 received in 2012-10-28, accepted in 2013-03-26,  发布年份 2013
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【 摘 要 】

Background

Petroselinum crispum, a bright green biennial shrub is widely used traditionally as a food additive and herbal remedies for many ailments. This study therefore aimed to assess the toxic effects of its leaf extract using some biochemical, haematological parameters.

Methods

The toxic effects were assessed by quantifying liver enzymes such as serum aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total serum protein and liver weight. Effects on haematological parameters were assessed by analysis of parked cell volume (PCV), red blood cell count (RBC), white blood cells (WBC) and haemoglobin (Hb) concentrations. Histopathological studies were done on the liver and kidneys.

Results

The extract caused significant increase in serum activity of alanine amino transferase and blood urea nitrogen (BUN) levels at the dose of 1000 mg/kg. Other biochemical and haematological parameters were not affected at lower doses. Conversely, the liver weight was not affected after eight weeks of treatment at the dose levels studied. The organs obtained for pathological study, were structurally unchanged under histopathological evaluation at lower doses but inflammatory and necrotic features were observed at doses ≥ 1000 mg/kg.

Conclusion

The results indicate that the leaf ethanol extract of Petroselinum crispum was hepatotoxic and nephrotoxic at continued oral doses equal to or more than 1000 mg/kg, but no obvious toxicity when used at lower doses. Therefore, there should be caution in its administration to avoid overdosing and known interaction with some medications. In addition, the plant should be kept away from pets and domestic animals and should not be cultivated on soil irrigated with waste water due to their ability to bio-accumulate toxic metals.

【 授权许可】

   
2013 Awe and Banjoko; licensee BioMed Central Ltd.

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【 参考文献 】
  • [1]Maria L: Aromatherapy Science: In a guide for health care professionals. Wallingford: CABI Publishing: Medical Pharmaceutical Press; 2006:462.
  • [2]Steve C: Petroselinium crispum. 2000. http://www.floridata.com/Petroseliniu.crispum.html webcite. Last assessed 08-08-2012
  • [3]Simmon JE, Overly ME: A comparative evaluation of Petroselinium cultivars. The spice and Medicinal Plant Digest 1986, 4(1):3-47.
  • [4]Parsley effectiveness, how it works and drug interaction. http://www.emedicine.health.com webcite. Last assessed 07-02-2013
  • [5]Properties of Parsley. http://www.botanical-online.com/medicinals webcite. Last assessed 07-02-2013
  • [6]Kreydiyyeh SJ, Usta J: Diuretic effect and mechanism of action of Parsley. J Ethnopharmacol 2002, 79:353-359.
  • [7]Parsley and informed farmers. http://www.informedfarmers.com webcite. Last assessed 01-02-2013
  • [8]Parsley and Warfarin. http://www.ptinr.com//parsleyand webcite warfarin. Last assessed 01-02-2013
  • [9]Parsley information from drugs. http://www.drugs.com webcite. Last assessed 01-02-201310
  • [10]Jakovljvic V, Raskovic A, Popovic M, Sabo J: The effect of Celery and Parsley juice on pharmacodynamic activity of drugs involving cytochrome P450 in their metabolism. Eur J Drug Metab Pharmacokinet 2002, 27:153-156.
  • [11]ASPCA spring parsley. http://www.aspca.org webcite. Last assessed 02-02-2013
  • [12]Keser G, Buyuk G: Effects of waste water irrigation on chemical and physical properties of Petroselinum capsum. Biol Trace Elem Res 2012, 143(6):369-379.
  • [13]Zhang H, Chen F, Wang X, Yao H-Y: Evaluation of antioxidant activity of Parsley (Petroselinum crispum) essential oil and identification of its antioxidant constituents. Food Res Int 2006, 39:833-839.
  • [14]Zaynoun S, Abi-Ali L, Tenekjian K, Karban A: The bergapten content of garden parsley and its significance in causing cutaneous photosenstization. Clin Exp Dermatol 1985, 10:328-331.
  • [15]Manderfield MM, Schafer HW, Davidson PM, Zottola EA: Isolation and Identification of antimicrobial furocoumarins from parsley. J Food Prot 1997, 60:72-77.
  • [16]Yoshikawa M, Uemura T, Shimoda H, Kishi A, Kawahara Y, Mastuda H: Medicinal food stuff XVIII. Phytoestrogens from aerial part of Petroselinium crispum Mill, and structures of 6”-acetylopin and a new monoterpene glycoside. Chem Pharm Bull 2000, 48:1039-1044.
  • [17]Popovic D, Kaurinovic B, Jakovljvic V, Mimica-Dukic N, Bursac M: Effect of Petroselinium crispum extracts on some biochemical parameters of oxidase stress in mice treated with carbon tetrachloride. Phytothera Res 2007, 7:531-537.
  • [18]Nehal M, Belal A: Hepatoprotective effect of feeding celery leaves mixed with Chicory leaves and Barley Grains to hypercholesterolemic rats. Asian J Clin Nutrition 2011, 3:14-24.
  • [19]Stone SH: Method for obtaining venous blood from orbital sinus of rat or mouse. Science 1954, (119):100.
  • [20]Riley V: Adaptation of orbital bleeding technique to rapid serial blood studies. Pro Soc Exp Biol Med 1960, 104:751-754.
  • [21]Ogbu SH, Okechukwu FJ: The effect of storage temperature prior to separation of plasma and serum protein. J Med Lab Sc 2001, 10:1-4.
  • [22]Reitman S, Frankel S: A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvate transaminases. Am J Clin Path 1957, 28:56-63.
  • [23]Gesellschaft K: Alkaline phosphatise. Chem Klin Biochem 1972, 10:182-192.
  • [24]Coles EH: Veterinary Clinical Pathology. 3rd edition. Philadephia: W.B Saunders & Co; 1986:192-222.
  • [25]Doumas BT, Watson WA, Biggs HG: Albumin standards and the measurement of serum albumin with bromocresol green. Clin Chem Acta 1971, 31:87-96.
  • [26]Fawcett JK, Scott JE: A rapid and precise method for the determination of urea. J Clin Path 1960, 13:156-159.
  • [27]Carleton H: Histological techniques. 4th edition. London: Oxford University Press; 1980. New York, USA
  • [28]Malomo AO: Toxicological implications of certriaxone administration in rats. Nig J Biochem Mol Biol 2000, 1533-1538.
  • [29]Larrey D: Epidemiology and individual susceptibility to adverse drug reactions affecting the liver. Semin Liver Dis 2002, 22:145-155.
  • [30]Martins AC: Clinical chemistry and metabolic medicine. 7th edition. UK: Edward Arnold Ltd; 2006:7-15.
  • [31]Kaneko H: Clinical biochemistry of domestic animals. San Diego: Academic; 1989.
  • [32]Trauner M, Meier PJ, Boyer JL: Molecular pathogenesis of cholestasis. N Eng J Med 1998, 339:1217-1227.
  • [33]Ochei J, Kolhatkar A: Medical laboratory science. Theory and practice. 2nd edition. New Delhi: Tata McGraw- Hill; 2000:331-349.
  • [34]Report on Parsley side effects and usage. http://www.askdoc.wsb.com/parsley.html webcite. Last assessed 10-02-2013
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