Transcriptome profiling of Bacillus subtilis OKB105 in response to rice seedlings

BMC Microbiology

Xuewen Gao¹  Yongli Xie¹  Haoyu Zang¹  Lina Chen¹  Huijun Wu¹  Shanshan Xie¹ 

[1]Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Weigang No.1, Nanjing 210095, People’s Republic of China

关键词: Functional annotation;    Microarray;    Transcriptomics;    Plant-microbe interactions;    Oryza sativa;    Bacillus subtilis;   

Others  :  1137441
DOI  :  10.1186/s12866-015-0353-4

 received in 2014-03-31, accepted in 2015-01-19,  发布年份 2015

【 摘 要 】

Background

Plant growth-promoting rhizobacteria (PGPR) are soil beneficial microorganisms that colonize plant roots for nutritional purposes and accordingly benefit plants by increasing plant growth or reducing disease. However, the mechanisms and pathways involved in the interactions between PGPR and plants remain unclear. In order to better understand these complex plant-PGPR interactions, changes in the transcriptome of the typical PGPR Bacillus subtilis in response to rice seedlings were analyzed.

Results

Microarray technology was used to study the global transcriptionl response of B. subtilis OKB105 to rice seedlings after an interaction period of 2 h. A total of 176 genes representing 3.8% of the B. subtilis strain OKB105 transcriptome showed significantly altered expression levels in response to rice seedlings. Among these, 52 were upregulated, the majority of which are involved in metabolism and transport of nutrients, and stress responses, including araA, ywkA, yfls, mtlA, ydgG et al. The 124 genes that were downregulated included cheV, fliL, spmA and tua, and these are involved in chemotaxis, motility, sporulation and teichuronic acid biosynthesis, respectively.

Conclusions

We present a transcriptome analysis of the bacteria Bacillus subtilis OKB105 in response to rice seedings. Many of the 176 differentially expressed genes are likely to be involved in the interaction between Gram-positive bacteria and plants.

【 授权许可】

   
2015 Xie et al.; licensee BioMed Central.

【 图 表 】

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