Influence of light-curing mode on the cytotoxicity of resin-based surface sealants

BMC Oral Health

Georgios N Belibasakis²  Thomas Attin¹  Tobias T Tauböck¹  Florian J Wegehaupt¹ 

[1] Clinic for Preventive Dentistry, Periodontology and Cariology, University of Zurich, Plattenstrasse 11, 8032 Zürich, Switzerland;Section of Oral Microbiology and Immunology, Institute of Oral Biology, University of Zürich, Plattenstrasse 11, 8032 Zürich, Switzerland

关键词: Cytotoxicity;    Resin;    Light exposure time;    Surface sealant;    Dentine;   

Others  :  1109772
DOI  :  10.1186/1472-6831-14-48

 received in 2014-01-15, accepted in 2014-05-01,  发布年份 2014

【 摘 要 】

Background

Surface sealants have been successfully used in the prevention of erosive tooth wear. However, when multiple tooth surfaces should be sealed, the light-curing procedure is very time-consuming. Therefore, the aim of this study was to investigate whether reduced light-curing time (while maintaining similar energy density) has an influence on resin-based surface sealant cytotoxicity.

Methods

Bovine dentine discs were treated as follows: group 1: untreated, groups 2–5: Seal&Protect and groups 6–9: experimental sealer. Groups 2 and 6 were light-cured (VALO LED light-curing device) for 40 s (1000 mW/cm²), groups 3 and 7 for 10 s (1000 mW/cm²), groups 4 and 8 for 7 s (1400 mW/cm²) and groups 5 and 9 for 3 s (3200 mW/cm²). Later, materials were extracted in culture medium for 24 h, and released lactate dehydrogenase (LDH) activity as a measure of cytotoxicity was determined photometrically after cells (dental pulp cells and gingival fibroblasts) were exposed to the extracts for 24 h. Three independent experiments, for both sample preparation and cytotoxicity testing, were performed.

Results

Overall, lowest cytotoxicity was observed for the unsealed control group. No significant influence of light-curing settings on the cytotoxicity was observed (p = 0.537 and 0.838 for pulp cells and gingival fibroblasts, respectively). No significant difference in the cytotoxicity of the two sealants was observed after light-curing with same light-curing settings (group 2 vs. 6, 3 vs. 7, 4 vs. 8 and 5 vs. 9: p > 0.05, respectively).

Conclusions

Shortening the light-curing time, while maintaining constant energy density, resulted in no higher cytotoxicity of the investigated sealants.

【 授权许可】

   
2014 Wegehaupt et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Wegehaupt FJ, Attin T: The role of fluoride and casein phosphopeptide/amorphous calcium phosphate in the prevention of erosive/abrasive wear in an in vitro model using hydrochloric acid. Caries Res 2010, 44:358-363.
• [2]Ganss C, Schlueter N, Hardt M, Schattenberg P, Klimek J: Effect of fluoride compounds on enamel erosion in vitro: a comparison of amine, sodium and stannous fluoride. Caries Res 2008, 42:2-7.
• [3]Brunton PA, Kalsi KS, Watts DC, Wilson NH: Resistance of two dentin-bonding agents and a dentin densensitizer to acid erosion in vitro. Dent Mater 2000, 16:351-355.
• [4]Azzopardi A, Bartlett DW, Watson TF, Sherriff M: The measurement and prevention of erosion and abrasion. J Dent 2001, 29:395-400.
• [5]Bartlett D, Sundaram G, Moazzez R: Trial of protective effect of fissure sealants, in vivo, on the palatal surfaces of anterior teeth, in patients suffering from erosion. J Dent 2011, 39:26-29.
• [6]Sundaram G, Wilson R, Watson TF, Bartlett D: Clinical measurement of palatal tooth wear following coating by a resin sealing system. Oper Dent 2007, 32:539-543.
• [7]Wegehaupt FJ, Tauböck TT, Sener B, Attin T: Long-term protective effect of surface sealants against erosive wear by intrinsic and extrinsic acids. J Dent 2012, 40:416-422.
• [8]Wegehaupt FJ, Taubock TT, Sener B, Attin T: Influence of light-curing mode on the erosion preventive effect of three different resin-based surface sealants. Int J Dent 2012, 2012:874359.
• [9]Ferreira SQ, Costa TR, Klein-Junior CA, Accorinte M, Meier MM, Loguercio AD, Reis A: Improvement of exposure times: effects on adhesive properties and resin-dentin bond strengths of etch-and-rinse adhesives. J Adhes Dent 2011, 13:235-241.
• [10]Reis A, Ferreira SQ, Costa TR, Klein-Junior CA, Meier MM, Loguercio AD: Effects of increased exposure times of simplified etch-and-rinse adhesives on the degradation of resin-dentin bonds and quality of the polymer network. Eur J Oral Sci 2010, 118:502-509.
• [11]Scotti N, Venturello A, Migliaretti G, Pera F, Pasqualini D, Geobaldo F, Berutti E: New-generation curing units and short irradiation time: the degree of conversion of microhybrid composite resin. Quintessence Int 2011, 42:e89-e95.
• [12]Tauböck TT, Bortolotto T, Buchalla W, Attin T, Krejci I: Influence of light-curing protocols on polymerization shrinkage and shrinkage force of a dual-cured core build-up resin composite. Eur J Oral Sci 2010, 118:423-429.
• [13]Ergun G, Egilmez F, Yilmaz S: Effect of reduced exposure times on the cytotoxicity of resin luting cements cured by high-power led. J Appl Oral Sci 2011, 19:286-292.
• [14]de Souza Costa CA, Hebling J, Hanks CT: Effects of light-curing time on the cytotoxicity of a restorative resin composite applied to an immortalized odontoblast-cell line. Oper Dent 2003, 28:365-370.
• [15]Feng L, Carvalho R, Suh BI: Insufficient cure under the condition of high irradiance and short irradiation time. Dent Mater 2009, 25:283-289.
• [16]Hadis M, Leprince JG, Shortall AC, Devaux J, Leloup G, Palin WM: High irradiance curing and anomalies of exposure reciprocity law in resin-based materials. J Dent 2011, 39:549-557.
• [17]Ferracane JL, Greener EH: The effect of resin formulation on the degree of conversion and mechanical properties of dental restorative resins. J Biomed Mater Res 1986, 20:121-131.
• [18]Caughman WF, Caughman GB, Shiflett RA, Rueggeberg F, Schuster GS: Correlation of cytotoxicity, filler loading and curing time of dental composites. Biomaterials 1991, 12:737-740.
• [19]Jagdish N, Padmanabhan S, Chitharanjan AB, Revathi J, Palani G, Sambasivam M, Sheriff K, Saravanamurali K: Cytotoxicity and degree of conversion of orthodontic adhesives. Angle Orthod 2009, 79:1133-1138.
• [20]Franz A, Konig F, Lucas T, Watts DC, Schedle A: Cytotoxic effects of dental bonding substances as a function of degree of conversion. Dent Mater 2009, 25:232-239.
• [21]Ulker HE, Hiller KA, Schweikl H, Seidenader C, Sengun A, Schmalz G: Human and bovine pulp-derived cell reactions to dental resin cements. Clin Oral Investig 2012, 16:1571-1578.
• [22]Belibasakis GN, Bostanci N, Hashim A, Johansson A, Aduse-Opoku J, Curtis MA, Hughes FJ: Regulation of RANKL and OPG gene expression in human gingival fibroblasts and periodontal ligament cells by Porphyromonas gingivalis: a putative role of the Arg-gingipains. Microb Pathog 2007, 43:46-53.
• [23]Belibasakis GN, Meier A, Guggenheim B, Bostanci N: Oral biofilm challenge regulates the RANKL-OPG system in periodontal ligament and dental pulp cells. Microb Pathog 2011, 50:6-11.
• [24]Koulaouzidou EA, Helvatjoglu-Antoniades M, Palaghias G, Karanika-Kouma A, Antoniades D: Cytotoxicity evaluation of an antibacterial dentin adhesive system on established cell lines. J Biomed Mater Res B Appl Biomater 2008, 84:271-276.
• [25]Koulaouzidou EA, Helvatjoglu-Antoniades M, Palaghias G, Karanika-Kouma A, Antoniades D: Cytotoxicity of dental adhesives in vitro. Eur J Dent 2009, 3:3-9.
• [26]Sigusch BW, Pflaum T, Volpel A, Schinkel M, Jandt KD: The influence of various light curing units on the cytotoxicity of dental adhesives. Dent Mater 2009, 25:1446-1452.
• [27]Porto IC, Andrade AK, Guenes GM, Ribeiro AI, Braz R, Castro CM: In vitro potential cytotoxicity of an adhesive system to alveolar macrophages. Braz Dent J 2009, 20:195-200.
• [28]Tang AT, Li J, Ekstrand J, Liu Y: Cytotoxicity tests of in situ polymerized resins: methodological comparisons and introduction of a tissue culture insert as a testing device. J Biomed Mater Res 1999, 45:214-222.
• [29]Chai Z, Li F, Fang M, Wang Y, Ma S, Xiao Y, Huang L, Chen J: The bonding property and cytotoxicity of a dental adhesive incorporating a new antibacterial monomer. J Oral Rehabil 2011, 38:849-856.
• [30]Demirci M, Hiller KA, Bosl C, Galler K, Schmalz G, Schweikl H: The induction of oxidative stress, cytotoxicity, and genotoxicity by dental adhesives. Dent Mater 2008, 24:362-371.
• [31]Oesterle LJ, Shellhart WC, Belanger GK: The use of bovine enamel in bonding studies. Am J Orthod Dentofacial Orthop 1998, 114:514-519.
• [32]Krifka S, Seidenader C, Hiller KA, Schmalz G, Schweikl H: Oxidative stress and cytotoxicity generated by dental composites in human pulp cells. Clin Oral Investig 2012, 16:215-224.
• [33]Yano J, Kitamura C, Nishihara T, Tokuda M, Washio A, Chen KK, Terashita M: Apoptosis and survivability of human dental pulp cells under exposure to Bis-GMA. J Appl Oral Sci 2011, 19:218-222.
• [34]Urcan E, Scherthan H, Styllou M, Haertel U, Hickel R, Reichl FX: Induction of DNA double-strand breaks in primary gingival fibroblasts by exposure to dental resin composites. Biomaterials 2010, 31:2010-2014.
• [35]Wroblewski F, LaDue JS: Lactic dehydrogenase activity in blood. Proc Soc Exp Biol Med 1955, 90:210-213.
• [36]Belibasakis G, Johansson A, Wang Y, Claesson R, Chen C, Asikainen S, Kalfas S: Inhibited proliferation of human periodontal ligament cells and gingival fibroblasts by Actinobacillus actinomycetemcomitans: involvement of the cytolethal distending toxin. Eur J Oral Sci 2002, 110:366-373.
• [37]Porto IC, Oliveira DC, Raele RA, Ribas KH, Montes MA, De Castro CM: Cytotoxicity of current adhesive systems: in vitro testing on cell cultures of primary murine macrophages. Dent Mater 2011, 27:221-228.
• [38]Wiegand A, Caspar C, Becker K, Werner C, Attin T: In vitro cytotoxicity of different self-etching dental adhesive systems. Schweiz Monatsschr Zahnmed 2006, 116:614-621.
• [39]Wegehaupt FJ, Taubock TT, Attin T: Influence of prophylaxis paste treatment on the abrasive wear of surface sealants. Acta Odontol Scand 2013, 71:744-750.
• [40]Azzopardi A, Bartlett DW, Watson TF, Sherriff M: The surface effects of erosion and abrasion on dentine with and without a protective layer. Br Dent J 2004, 196:351-354. discussion 339
• [41]Kookana RS, Ying GG, Waller NJ: Triclosan: its occurrence, fate and effects in the Australian environment. Water Sci Technol 2011, 63:598-604.
• [42]D‘Arezzo S, Lanini S, Puro V, Ippolito G, Visca P: High-level tolerance to triclosan may play a role in pseudomonas aeruginosa antibiotic resistance in immunocompromised hosts: evidence from outbreak investigation. BMC Res Notes 2012, 5:43. BioMed Central Full Text
• [43]Adolfsson-Erici M, Pettersson M, Parkkonen J, Sturve J: Triclosan, a commonly used bactericide found in human milk and in the aquatic environment in Sweden. Chemosphere 2002, 46:1485-1489.