High risk human papillomavirus and Epstein Barr virus in human breast milk

BMC Research Notes

James S Lawson¹  Noel J Whitaker¹  Wendy K Glenn¹ 

[1] School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia

关键词: Human milk;    Epstein barr virus;    Human papillomavirus;   

Others  :  1165800
DOI  :  10.1186/1756-0500-5-477

 received in 2012-04-30, accepted in 2012-08-30,  发布年份 2012

【 摘 要 】

Background

Multiple viruses, including human immunodeficiency virus, Epstein Barr virus (EBV) and mouse mammary tumour virus have been identified in human milk. High risk human papillomavirus (HPV) sequences have been identified in breast cancer. The aim of this study is to determine if viral sequences are present in human milk from normal lactating women.

Findings

Standard (liquid) and in situ polymerase chain reaction (PCR) techniques were used to identify HPV and EBV in human milk samples from normal lactating Australian women who had no history of breast cancer.

High risk human papillomavirus was identified in milk samples of 6 of 40 (15%) from normal lactating women - sequencing on four samples showed three were HPV 16 and one was HPV 18. Epstein Barr virus was identified in fourteen samples (33%).

Conclusion

The presence of high risk HPV and EBV in human milk suggests the possibility of milk transmission of these viruses. However, given the rarity of viral associated malignancies in young people, it is possible but unlikely, that such transmission is associated with breast or other cancers.

【 授权许可】

   
2012 Glenn et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Kjaer SK, Chackerian B, van den Brule AJ, Svare EI, Paull G, Walbomers JM, Schiller JT, Bock JE, Sherman ME, Lowy DR, Meijer CL: High-risk human papillomavirus is sexually transmitted;evidence from a follow-up study of virgins starting sexual activity (intercourse). Cancer Epidemiol Biomarkers Prev 2001, 10:101-116.
• [2]Chen AC, Keleher A, Kedda MA, Spurdle AB, McMillan NA, Antonsson A: Human papillomavirus: DNA detected in peripheral blood samples from healthy Australian male blood donors. J Med Virol 2009, 81:1792-1796.
• [3]Rautava J, Syrjänen S: Human papillomavirus infections in the oral mucosa. J Am Dent Assoc 2011, 142:905-914.
• [4]Syrjänen S: Current concepts on human papillomavirus infections in children. APMIS 2010, 118:494-509.
• [5]Sarkola M, Funtala M, Grenman S, Syganen S: Human papillomavirus DNA detected in breast milk. Ped Inf Dis J 2008, 27:557-558.
• [6]Cazzaniga M, Gheit T, Casadio C, Kahn N, Macis D, Valenti F, Miller MJ, Sylla BS, Akiba S, Bonnani B, Decensi A, Veronesi V, Tommasino M: Analysis of the presence of cutaneous and mucosal papilloma virus types in ductal lavage fluid, milk, and colostrum to evaluate its role in breast carcinogenesis. Breast Cancer Res Treat 2009, 114:599-605.
• [7]Mammas IN, Zaravinos A, Sourvinos G, Myriokefalitakis N, Theodoridou M, Spandidos DA: Can 'high-risk' human papillomaviruses (HPVs) be detected in human breast milk? Acta Paediatr 2011, 100:705-707.
• [8]Hagensee ME, Herrel N, D’Angelo A, Sutton K, Clark R, Cameron JE: Epstein Barr virus interacts with human papilloma virus to decrease apoptosis in vitro and increase cervical dysplasia in vivo. International Centre Genetic Engineering Biotechnology DNA Tumour virus Meeting proceedings, Trieste; 2011. Personal communication. Email for Jennifer Cameron: [e-mail]
• [9]Glenn WK, Heng B, Whitaker NJ, Lawson JS: Letter to the Editor Reply: Koilocytes indicate a role for human papilloma virus in breast milk. Br J Cancer 2010, 102:788-788.
• [10]Heng B, Glenn WK, Ye Y, Tran D, Delprado W, Lutze-Mann L, Whitaker NJ, Lawson JS: Human papilloma virus is associated with breast cancer. Br J Cancer 2009, 101:1345-1350.
• [11]Cinque P, Brytting M, Vago L, Castagna A, Parravicini C, Zanchetta N, D'Arminio Monforte A, Wahren B, Lazzarin A, Linde A: Epstein-Barr virus DNA in cerebrospinal fluid from patients with AIDS-related primary lymphoma of the central nervous system. Lancet 1993, 342:398-401.
• [12]Coovadia HM, Rollins NC, Bland RM, Little K, Coutsoudis A, Bennish ML, Newell M-L: Mother-to-child transmission of HIV-1 infection during exclusive breastfeeding in the first 6 months of life: an intervention cohort study. Lancet 2007, 369:1107-1116.
• [13]Johal H, Ford CE, Glenn WK, Heads J, Lawson JS, Rawlinson WD: Mouse mammary tumor like virus (MMTV) sequences in breast milk from healthy lactating women. Breast Cancer Res Treat 2011, 129:149-155.
• [14]Junker AK, Thomas EE, Radcliffe A, Forsyth RB, Davidson AGF, Rymao L: Epstein-Barr virus shedding in breast milk. Am J Med Sci 1991, 302:220-223.
• [15]Puranen MH, Yliskoski MH, Saarikoski SV, Syrjanen KJ, Syrjanen SM: Exposure of an infant to cervical human papillomavirus infection of the mother is common. Am J Obstet Gynecol 1997, 176:1039-1045.
• [16]Gravitt PE: The known unknowns of HPV natural history. J Clin Invest 2011, 121:4593-4599.