Genome-scale analysis of the high-efficient protein secretion system of Aspergillus oryzae

BMC Systems Biology

Jens Nielsen¹  Carsten Hjort²  Tobias Österlund³  Amir Feizi³  Lifang Liu³ 

[1] Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Fremtidsvej 3, DK-2970 Hørsholm, Denmark;Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Denmark;Novo Nordisk Foundation Center for Biosustainability, Department of Chemical and Biologicl Engineering, Chalmers University of Technology, SE-41296 Göteborg, Sweden

关键词: Transcriptome;    Secretory pathway;    Comparative genomics;    α-amylase;    Aspergillus oryzae;   

Others  :  864930
DOI  :  10.1186/1752-0509-8-73

 received in 2013-12-04, accepted in 2014-06-18,  发布年份 2014

【 摘 要 】

Background

The koji mold, Aspergillus oryzae is widely used for the production of industrial enzymes due to its particularly high protein secretion capacity and ability to perform post-translational modifications. However, systemic analysis of its secretion system is lacking, generally due to the poorly annotated proteome.

Results

Here we defined a functional protein secretory component list of A. oryzae using a previously reported secretory model of S. cerevisiae as scaffold. Additional secretory components were obtained by blast search with the functional components reported in other closely related fungal species such as Aspergillus nidulans and Aspergillus niger. To evaluate the defined component list, we performed transcriptome analysis on three α-amylase over-producing strains with varying levels of secretion capacities. Specifically, secretory components involved in the ER-associated processes (including components involved in the regulation of transport between ER and Golgi) were significantly up-regulated, with many of them never been identified for A. oryzae before. Furthermore, we defined a complete list of the putative A. oryzae secretome and monitored how it was affected by overproducing amylase.

Conclusion

In combination with the transcriptome data, the most complete secretory component list and the putative secretome, we improved the systemic understanding of the secretory machinery of A. oryzae in response to high levels of protein secretion. The roles of many newly predicted secretory components were experimentally validated and the enriched component list provides a better platform for driving more mechanistic studies of the protein secretory pathway in this industrially important fungus.

【 授权许可】

   
2014 Liu et al.; licensee BioMed Central Ltd.

【 图 表 】

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