Effect of green tea extracts on oxaliplatin-induced peripheral neuropathy in rats

BMC Complementary and Alternative Medicine

Yoon Ho Ko²  Young-Seok Cho¹  Hye Sung Won²  Eun Kyoung Jeon²  Yoon Tae Kim³  Jung Soo Lee³ 

[1] Division of Gastroenterology, Department of Internal Medicine, Uijeongbu St. Mary's hospital, The Catholic University of Korea, Uijeongbu, South Korea;Division of Oncology, Department of Internal Medicine, Uijeongbu St. Mary's hospital, The Catholic University of Korea, 65-1, Geumo-dong, Uijeongbu- si, 480-717, South Korea;Department of Rehabilitation Medicine, Uijeongbu St. Mary's hospital, The Catholic University of Korea, Uijeongbu, South Korea

关键词: Antioxidant;    Green tea extract;    Peripheral neuropathy;    Oxaliplatin;   

Others  :  1232091
DOI  :  10.1186/1472-6882-12-124

 received in 2011-09-25, accepted in 2012-08-05,  发布年份 2012

【 摘 要 】

Background

A common side effect of oxaliplatin is peripheral neurotoxicity. Oxidative stress to dorsal root ganglion (DRG) may be one of important pathogenic mechanisms. Green tea contains four polyphenol catechins, which are known to be potent antioxidants. The present work is aimed to determine whether green tea extracts have neuroproective or palliative effects on neurotoxicity symptoms induced by oxaliplatin.

Methods

We conducted behavioral tests including sensory and thermal thresholds, an electrophysiological study, and TUNEL staining to assess neurotoxicity during the experimental period using animal models.

Results

A total of 14 adult rats were randomly allocated into two groups. Oxaliplatin (4 mg/kg) with or without green tea (300 mg/kg orally once daily) was administered intraperitoneally twice per week for 6 weeks. At 4 and 6 weeks after oxaliplatin administration, sensory threshold values were significantly decreased and at 6 weeks after oxaliplatin administration, thermal threshold values were significantly increased in oxaliplatin-treated rats compared with those in rat treated with oxaliplatin and green tea extracts. The electrophysiological assessment, including sensory nerve conduction and H-reflex-related sensory nerve conduction velocity, revealed no significant changes in the two groups. TUNEL staining showed no significant difference in the number of apoptotic-featured cells between the two experimental groups in the DRG or peripheral nerves, but the number of apoptotic-featured cells in DRG was higher than that in sciatic nerves within each group.

Conclusions

Green tea extracts may be a useful adjuvant to alleviate sensory symptoms after oxaliplatin administration, such as allodynia, but did not prevent morphometric or electrophysiological alterations induced by oxaliplatin.

【 授权许可】

   
2012 Lee et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

【 参考文献 】

• [1]Argyriou AA, Polychronopoulos P, Iconomou G, Chroni E, Kalofonos HP: A review on oxaliplatin-induced peripheral nerve damage. Cancer Treat Rev 2008, 34:368-377.
• [2]Grothey A: Clinical management of oxaliplatin-associated neurotoxicity. Clin Colorectal Cancer 2005, 5(Suppl 1):38-46.
• [3]Lehky T, Leonard GD, Wilson RH, Grem JL, Floeter MK: Oxaliplatin induced neurotoxicity: Acute hyperexcitability and chronic neuropathy. Muscle Nerve 2004, 29:387-392.
• [4]Park SB, Goldstein D, Lin CS, Krishnan AV, Friedlander ML, Kiernan MC: Acute abnormalities of sensory nerve function associated with oxaliplatin-induced neurotoxicity. J Clin Oncol 2009, 27:1243-1249.
• [5]Cavaletti G, Tredici G, Petruccioli MG, Dondè E, Tredici P, Marmiroli P, Minoia C, Ronchi A, Bayssas M, Etienne GG: Effects of different schedules of oxaliplatin treatment on the peripheral nervous system of the rat. Eur J Cancer 2001, 37:2457-2463.
• [6]Kann O, Kovacs R: Mitochondria and neuronal activity. Am J Physiol Cell Physiol 2007, 292:C641-C657.
• [7]Lersch C, Schmelz R, Eckel F, Erdmann J, Mayr M, Schulte-Frohlinde E, Quasthoff S, Grosskreutz J, Adelsberger H: Prevention of oxaliplatin-induced peripheral sensory neuropathy by carbamazepine in patients with advanced colorectal cancer. Clin Colorectal Cancer 2002, 2:54-58.
• [8]Weijl NI, Hopman GD, Wipkink-Bakker A, Lentjes EG, Berger HM, Cleton FJ, Osanto S: Cisplatin combination chemotherapy induces a fall in plasma antioxidants of cancer patients. Ann Oncol 1998, 9:1331-1337.
• [9]Gamelin E, Gamelin L, Bossi L, Quasthoff S: Clinical aspects and molecular basis of oxaliplatin neurotoxicity: current management and development of preventive measures. Semin Oncol 2002, 29(5 Suppl 15):21-33.
• [10]Krishnan AV, Goldstein D, Friedlander M, Kiernan MC: Oxaliplatin-induced neurotoxicity and the development of neuropathy. Muscle Nerve 2005, 32:51-60.
• [11]Carozzi VA, Marmiroli P, Cavaletti G: The Role of Oxidative Stress and Anti-Oxidant Treatment in Platinum-Induced Peripheral Neurotoxicity. Curr Cancer Drug Targets 2010, 10:670-682.
• [12]McWhinney SR, Goldberg RM, McLeod HL: Platinum neurotoxicity pharmacogenetics. Mol Cancer Ther 2009, 8:10-16.
• [13]Nanjo F, Mori M, Goto K, Hara Y: Radical scavenging activity of tea catechins and their related compounds. Biosci Biotechnol Biochem 1999, 63:1621-1623.
• [14]Zaveri NT: Green tea and its polyphenolic catechins: medicinal uses in cancer and noncancer applications. Life Sci 2006, 78:2073-2080.
• [15]Pace A, Savarese A, Picardo M, Maresca V, Pacetti U, Del Monte G, Biroccio A, Leonetti C, Jandolo B, Cognetti F, Bove L: Neuroprotective effect of vitamin E supplementation in patients treated with cisplatin chemotherapy. J Clin Oncol 2003, 21:927-931.
• [16]Joseph EK, Chen X, Bogen O, Levine JD: Oxaliplatin acts on IB4-positive nociceptors to induce an oxidative stress-dependent acute painful peripheral neuropathy. J Pain 2008, 9:463-472.
• [17]Romeo L, Intrieri M, D'Agata V, Mangano NG, Oriani G, Ontario ML, Scapagnini G: The major green tea polyphenol,(-)-epigallocatechin-3-gallate, induces heme oxygenase in rat neurons and acts as an effective neuroprotective agent against oxidative stress. J Am Coll Nutr 2009, 28(Suppl 1):492S-499S.
• [18]De Grandis D: Acetyl-L-carnitine for the treatment of chemotherapy-induced peripheral neuropathy: a short review. CNS Drugs 2007, 21(Suppl 1):39-43.
• [19]Pisano C, Pratesi G, Laccabue D, Zunino F, Lo Giudice P, Bellucci A, Pacifici L, Camerini B, Vesci L, Castorina M, Cicuzza S, Tredici G, Marmiroli P, Nicolini G, Galbiati S, Calvani M, Carminati P, Cavaletti G: Paclitaxel and cisplatin-induced neurotoxicity. Clin Cancer Res 2003, 9:5756-5767.
• [20]Helgren ME, Cliffer KD, Torrento K, Cavnor C, Curtis R, DiStefano PS, Wiegand SJ, Lindsay RM: Neurotrophin-3 administration attenuates deficits of pyridoxine-induced large-fiber sensory neuropathy. J Neurosci 1997, 17:372-382.
• [21]Park SB, Lin CS, Krishnan AV, Goldstein D, Friedlander ML, Kiernan MC: Oxaliplatin-induced neurotoxicity: changes in axonal excitability precede development of neuropathy. Brain 2009, 132:271227-23.
• [22]Cascinu S, Catalano V, Cordella L, Labianca R, Giordani P, Baldelli AM, Beretta GD, Ubiali E, Catalano G: Neuroprotective effect of reduced glutathione on oxaliplatin-based chemotherapy in advanced colorectal cancer: a randomized, double-blind, placebo-controlled trial. J Clin Oncol 2002, 20:3478-3483.
• [23]Gedlicka C, Scheithauer W, Schüll B, Kornek GV: Effective treatment of oxaliplatin-induced cumulative polyneuropathy with alpha-lipoic acid. J Clin Oncol 2002, 20:3359-3361.
• [24]Sadzuka Y, Sugiyama T, Hirota S: Modulation of cancer chemotherapy by green tea. Clin Cancer Res 1998, 4:153-156.