Development of the follicular basement membrane during human gametogenesis and early folliculogenesis

BMC Developmental Biology

Susana M Chuva de Sousa Lopes²  Lucette A J van der Westerlaken⁵  Frans M Helmerhorst⁵  Carina G Hilders³  Leonie A Louwe⁵  Maria M Gomes Fernandes¹  Matthias S Roost¹  Ana de Melo Bernardo¹  Daniëlle B Klootwijk¹  Liesbeth van Iperen¹  A Marijne Heeren⁴ 

[1] Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, Leiden, 2333, ZC, The Netherlands;Department for Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, Ghent, 9000, Belgium;Department of Gynaecology, Reinier de Graaf Hospital, Reinier de Graaf 3-11, Delft, 2625, AD, The Netherlands;Current address: Department of Obstetrics and Gynaecology, VU University Medical Center, De Boelelaan 1118, Amsterdam, 1081, HZ, The Netherlands;Department of Gynaecology, Leiden University Medical Center, Albinusdreef 2, Leiden, 2300, RC, The Netherlands

关键词: Gonads;    Extracellular matrix;    Follicles;    Adult ovary;    Germ cells;    Human;   

Others  :  1089670
DOI  :  10.1186/s12861-015-0054-0

 received in 2014-07-07, accepted in 2015-01-13,  发布年份 2015

【 摘 要 】

Background

In society, there is a clear need to improve the success rate of techniques to restore fertility. Therefore a deeper knowledge of the dynamics of the complex molecular environment that regulates human gametogenesis and (early) folliculogenesis in vivo is necessary. Here, we have studied these processes focusing on the formation of the follicular basement membrane (BM) in vivo.

Results

The distribution of the main components of the extracellular matrix (ECM) collagen IV, laminin and fibronectin by week 10 of gestation (W10) in the ovarian cortex revealed the existence of ovarian cords and of a distinct mesenchymal compartment, resembling the organization in the male gonads. By W17, the first primordial follicles were assembled individually in that (cortical) mesenchymal compartment and were already encapsulated by a BM of collagen IV and laminin, but not fibronectin. In adults, in the primary and secondary follicles, collagen IV, laminin and to a lesser extent fibronectin were prominent in the follicular BM.

Conclusions

The ECM-molecular niche compartimentalizes the female gonads from the time of germ cell colonization until adulthood. This knowledge may contribute to improve methods to recreate the environment needed for successful folliculogenesis in vitro and that would benefit a large number of infertility patients.

【 授权许可】

   
2015 Heeren et al.; licensee BioMed Central.

【 图 表 】

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