Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary

Biological research: BR

Wenzhong Liu¹  Jianbo Yao¹  Xuejing Yu¹  Xiaolei Yao¹  Pengfei Li²  Lihua Lyu²  Zhiwei Zhu³  Jianshan Xie⁴ 

[1] College of Animal Science and Technology, Shanxi Agricultural University, Taigu, China;College of Life Science, Shanxi Agricultural University, Taigu, China;Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, USA;School of Basic Medical Sciences, Shanxi Medical University, Taiyuan, China

关键词: Hen;    Follicles;    CART;    Theca cell;    Granulosa cell;   

DOI  :  10.1186/s40659-017-0123-x

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulation of food intake, energy homeostasis, and reproduction. The hens egg-laying rate mainly depends on the developmental status of follicles, expression of CART have not been identified from hen follicles, the regulatory mechanisms of CART biological activities are still unknown. The objective of this study was to characterize the mRNA expression of CART in hen follicular granulosa cells and determine CART peptide localization and regulatory role during follicular development. Small white follicles (1–2 mm in diameter) were treated for RNA isolation; Small white follicles (1–2 mm in diameter) and large white follicles (4–6 mm in diameter) were treated for immunohistochemical localization and large white follicles (4–6 mm in diameter), small yellow follicles (6–8 mm in diameter), large yellow follicles (9–12 mm in diameter), mature follicles (F5, F4, F3, F2, F1, >12 mm in diameter) were treated for RNA isolation and Real time PCR. The results showed that full length of the CDS of hen CART was 336 bp encoding a 111 amino acid polypeptide. In the hen ovary, CART peptide was primarily localized to the theca layer, but not all, the oocyte and granulosa layer, with diffused, weaker staining than relative to the theca cell layer. Further, amount of CART mRNA was more (P 

【 授权许可】

CC BY