No association found between the detection of either xenotropic murine leukemia virus-related virus or polytropic murine leukemia virus and chronic fatigue syndrome in a blinded, multi-site, prospective study by the establishment and use of the SolveCFS BioBank

BMC Research Notes

Peter Gerondelis⁴  David A Wilfret⁴  Katja S Remlinger²  James R Brown⁵  Daniel L Peterson⁷  Charles W Lapp⁸  Nancy G Klimas⁶  Lucinda Bateman¹  K Kimberly McCleary³  Suzanne D Vernon³  David M Irlbeck⁴ 

[1] Fatigue Consultation Clinic, Salt Lake City, UT, USA;Statistical Consulting Group, GlaxoSmithKline, Research Triangle Park, NC, USA;The CFIDS Association of America, Charlotte, NC, USA;Division of Infectious Diseases, GlaxoSmithKline, Research Triangle Park, NC, USA;Department of Computational Biology, GlaxoSmithKline, Collegeville, PA, USA;Nova Southeastern University College of Osteopathic Medicine, Institute for Neuro-Immune Medicine, Miami, FL, USA;Sierra Internal Medicine Associates, Incline Village, NV, USA;Hunter-Hopkins Center, Charlotte, NC, USA

关键词: Detection;    PCR;    Retrovirus;    Polytropic murine leukemia virus;    Xenotropic murine virus-related virus;    Chronic fatigue syndrome;   

Others  :  1131749
DOI  :  10.1186/1756-0500-7-461

 received in 2014-01-31, accepted in 2014-07-03,  发布年份 2014

【 摘 要 】

Background

In 2009, a retrospective study reported the detection of xenotropic murine leukemia virus-related virus (XMRV) in clinical isolates derived from individuals with chronic fatigue syndrome or myalgic encephalomyelitis (CFS). While many efforts to confirm this observation failed, one report detected polytropic murine leukemia virus (pMLV), instead of XMRV. In both studies, Polymerase Chain Reaction (PCR)-based methods were employed which could provide the basis for the development of a practical diagnostic tool. To confirm these studies, we hypothesized that the ability to detect these viruses will not only depend upon the technical details of the methods employed but also on the criteria used to diagnose CFS and the availability of well characterized clinical isolates.

Methods

A repository of clinical isolates from geographically distinct sites was generated by the collection of fresh blood samples from well characterized CFS and healthy subjects. Molecular techniques were used to generate assay positive controls and to determine the lower limit of detection (LLOD) for murine retroviral and Intracisternal A particle (Cell 12(4):963-72, 1977) detection methods.

Results

We report the establishment of a repository of well-defined, clinical isolates from five, geographically distinct regions of the US, the comparative determination of the LLODs and validation efforts for the previously reported detection methods and the results of an effort to confirm the association of these retroviral signatures in isolates from individuals with CFS in a blinded, multi-site, prospective study. We detected various, murine retroviral DNA signatures but were unable to resolve a difference in the incidence of their detection between isolates from CFS (5/72; 6.7%) and healthy (2/37; 5.4%) subjects (Fisher’s Exact Test, p-value = 1). The observed sequences appeared to reflect the detection of endogenous murine retroviral DNA, which was not identical to either XMRV or pMLV.

Conclusions

We were unable to confirm a previously reported association between the detection of XMRV or pMLV sequences and CFS in a prospective, multi-site study. Murine retroviral sequences were detected at a low frequency that did not differ between CFS and control subjects. The nature of these sequences appeared to reflect the detection of pre-existing, endogenous, murine retroviral DNA in the PCR reagents employed.

【 授权许可】

   
2014 Irlbeck et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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