Genome sequence of Ensifer adhaerens OV14 provides insights into its ability as a novel vector for the genetic transformation of plant genomes

BMC Genomics

Ewen Mullins³  Toni Wendt¹  Christopher J Creevey²  Fiona Doohan⁴  Steven Rudder⁴ 

[1] Current address: Carlsberg Research Centre, Gamle Carlsberg Vej 4-10, 1799 Copenhagen V, Denmark;Current address: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Ceredigion SY23 3FL, UK;Department of Crop Science, Teagasc Crops Research Centre, Oak Park, Carlow, Ireland;UCD Earth Institute and UCD School of Biology and Environmental Sciences, University College Dublin, Belfield, Dublin 4, Ireland

关键词: Genome sequencing;    Agrobacterium tumefaciens;    Transformation;    Ensifer adhaerens;   

Others  :  1217529
DOI  :  10.1186/1471-2164-15-268

 received in 2013-07-31, accepted in 2014-03-19,  发布年份 2014

【 摘 要 】

Background

Recently it has been shown that Ensifer adhaerens can be used as a plant transformation technology, transferring genes into several plant genomes when equipped with a Ti plasmid. For this study, we have sequenced the genome of Ensifer adhaerens OV14 (OV14) and compared it with those of Agrobacterium tumefaciens C58 (C58) and Sinorhizobium meliloti 1021 (1021); the latter of which has also demonstrated a capacity to genetically transform crop genomes, albeit at significantly reduced frequencies.

Results

The 7.7 Mb OV14 genome comprises two chromosomes and two plasmids. All protein coding regions in the OV14 genome were functionally grouped based on an eggNOG database. No genes homologous to the A. tumefaciens Ti plasmid vir genes appeared to be present in the OV14 genome. Unexpectedly, OV14 and 1021 were found to possess homologs to chromosomal based genes cited as essential to A. tumefaciens T-DNA transfer. Of significance, genes that are non-essential but exert a positive influence on virulence and the ability to genetically transform host genomes were identified in OV14 but were absent from the 1021 genome.

Conclusions

This study reveals the presence of homologs to chromosomally based Agrobacterium genes that support T-DNA transfer within the genome of OV14 and other alphaproteobacteria. The sequencing and analysis of the OV14 genome increases our understanding of T-DNA transfer by non-Agrobacterium species and creates a platform for the continued improvement of Ensifer-mediated transformation (EMT).

【 授权许可】

   
2014 Rudder et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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Figure 3.

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