IgG, IgM and IgA antibodies against the novel polyprotein in active tuberculosis

BMC Infectious Diseases

Heqiu Zhang²  Xiaoguo Song²  Guohua Wang²  Russell A Nicholson¹  Li Zhang³  Ping Zhao⁴  Kun Chen²  Zhenhua Dai²  Shuang Qie²  Bingshui Xiu²  Xiqin Yang²  Xiaoyan Feng² 

[1] Department of Biological Sciences, Simon Fraser University, Burnaby, B.C V5A 1S6, Canada;Department of Bio-diagnosis, Beijing Institute of Basic Medical Sciences, Beijing 100850, China;Tianjin Haihe Hospital, Tianjin 300350, China;Chaoyang District Centre for Disease Control and Prevention, Beijing 100029, China

关键词: Polyprotein;    Serodiagnosis;    Tuberculosis;   

Others  :  1127517
DOI  :  10.1186/1471-2334-14-336

 received in 2013-12-02, accepted in 2014-06-12,  发布年份 2014

【 摘 要 】

Background

The present study was aimed to evaluate whether IgG, IgM and IgA antibodies levels detected against a novel Mycobacterium tuberculosis polyprotein 38 F-64 F (with 38 F being the abbreviation for 38kD-ESAT6-CFP10 and 64 F for Mtb8.4-MPT64-TB16.3-Mtb8) are suitable for diagnosing active tuberculosis, and for monitoring the efficacy of chemotherapy on TB patients.

Methods

In this study, a total of 371 active TB patients without treatment were selected and categorized into S+/C+ group (n = 143), S-/C+ group (n = 106) or S-/C- group (n = 122). A series of serum samples were collected from 82 active TB patients who had undergone anti-TB chemotherapy for 0–6 months at one month interval. Humoral responses (IgG, IgM and IgA) were determined for the novel Mycobacterium tuberculosis polyprotein using indirect ELISA methods in all of serum samples.

Results

For S+/C+, S-/C+ and S-/C- active tuberculosis patients before anti-TB chemotherapy, the sensitivities of tests based on IgG were 65.7%, 46.2% and 52.5% respectively; the sensitivities based on IgM were 21.7%, 24.5% and 18.9%; and the sensitivities based on IgA were 25.2%, 17.9% and 23.8%. By combination of three isotypes, for all active tuberculosis patients, the test sensitivity increased to 70.4% with the specificity being 91.5%. After anti-TB chemotherapy, there were no significant differences between groups with different courses of anti-TB chemotherapy.

Conclusions

The novel Mycobacterium tuberculosis polyprotein 38 F-64 F represents potential antigen suitable for measuring IgG, IgM and IgA antibodies. However, the serodiagnostic test based on the 38 F-64 F polyprotein appears unsuitable for monitoring the efficacy of chemotherapy.

【 授权许可】

   
2014 Feng et al.; licensee BioMed Central Ltd.

【 图 表 】

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