The human cardiac and skeletal muscle proteomes defined by transcriptomics and antibody-based profiling

BMC Genomics

Mathias Uhlén⁴  Fredrik Pontén³  Erik Larsson²  Jens Nielsen⁵  Adil Mardinoglu⁵  Leif Väremo⁵  Peipei Ping⁴  David A Liem⁴  Howard Choi⁴  Caroline Kampf³  Mikael Huss⁶  Malene Lindholm⁷  Carl Johan Sundberg⁷  Björn M Hallström¹  Linn Fagerberg¹  Jerker Linné³  Cecilia Lindskog³ 

[1] Science for Life Laboratory, KTH - Royal Institute of Technology, AlbaNova University Center, Stockholm, SE-171 21, Sweden;Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, SE-751 85, Sweden;Science for Life Laboratory, Dept of Immunology Genetics and Pathology, Uppsala University, Uppsala, SE-751 85, Sweden;NHLBI Proteomics Center at UCLA, Departments of Physiology and Medicine, Division of Cardiology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA;Department of Chemical and Biological Engineering, Chalmers University of Technology, Gothenburg, SE-412 58, Sweden;Science for Life Laboratory, Dept of Biochemistry and Biophysics, Stockholm University, Solna, SE-17121, Sweden;Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, SE-171 77, Sweden

关键词: Cardiac and skeletal muscle;    Proteome;    Transcriptome;   

Others  :  1219272
DOI  :  10.1186/s12864-015-1686-y

 received in 2014-08-18, accepted in 2015-06-05,  发布年份 2015

【 摘 要 】

Background

To understand cardiac and skeletal muscle function, it is important to define and explore their molecular constituents and also to identify similarities and differences in the gene expression in these two different striated muscle tissues. Here, we have investigated the genes and proteins with elevated expression in cardiac and skeletal muscle in relation to all other major human tissues and organs using a global transcriptomics analysis complemented with antibody-based profiling to localize the corresponding proteins on a single cell level.

Results

Our study identified a comprehensive list of genes expressed in cardiac and skeletal muscle. The genes with elevated expression were further stratified according to their global expression pattern across the human body as well as their precise localization in the muscle tissues. The functions of the proteins encoded by the elevated genes are well in line with the physiological functions of cardiac and skeletal muscle, such as contraction, ion transport, regulation of membrane potential and actomyosin structure organization. A large fraction of the transcripts in both cardiac and skeletal muscle correspond to mitochondrial proteins involved in energy metabolism, which demonstrates the extreme specialization of these muscle tissues to provide energy for contraction.

Conclusions

Our results provide a comprehensive list of genes and proteins elevated in striated muscles. A number of proteins not previously characterized in cardiac and skeletal muscle were identified and localized to specific cellular subcompartments. These proteins represent an interesting starting point for further functional analysis of their role in muscle biology and disease.

【 授权许可】

   
2015 Lindskog et al.

【 图 表 】

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