Clustering subspecies of Aeromonas salmonicida using IS630 typing

BMC Microbiology

Philippe Vanden Bergh¹  Joachim Frey¹  Nicole Studer¹ 

[1] Institute of Veterinary Bacteriology, University of Bern, Länggassstrasse 122, Bern, Switzerland

关键词: Pathoadaptation;    Salmonidae;    Tc1 Mariner transposon;    Subtyping;    IS element;    HCN-IS630-RFLP;    Aeromonas salmonicida;   

Others  :  1144420
DOI  :  10.1186/1471-2180-13-36

 received in 2012-11-19, accepted in 2013-02-05,  发布年份 2013

【 摘 要 】

Background

The insertion element IS630 found in Aeromonas salmonicida belongs to the IS630-Tc1-mariner superfamily of transposons. It is present in multiple copies and represents approximately half of the IS present in the genome of A. salmonicida subsp. salmonicida A449.

Results

By using High Copy Number IS630 Restriction Fragment Length Polymorphism (HCN-IS630-RFLP), strains of various subspecies of Aeromonas salmonicida showed conserved or clustering patterns, thus allowing their differentiation from each other. Fingerprints of A. salmonicida subsp. salmonicida showed the highest homogeneity while ‘atypical’ A. salmonicida strains were more heterogeneous. IS630 typing also differentiated A. salmonicida from other Aeromonas species. The copy number of IS630 in Aeromonas salmonicida ranges from 8 to 35 and is much lower in other Aeromonas species.

Conclusions

HCN-IS630-RFLP is a powerful tool for subtyping of A. salmonicida. The high stability of IS630 insertions in A. salmonicida subsp. salmonicida indicates that it might have played a role in pathoadaptation of A. salmonicida which has reached an optimal configuration in the highly virulent and specific fish pathogen A. salmonicida subsp. salmonicida.

【 授权许可】

   
2013 Studer et al.; licensee BioMed Central Ltd.

【 图 表 】

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